##gff-version 3
##sequence-region BBa_I20250 1 903
BBa_I20250	.	engineered_region	775	854	.	+	0	ID=annotation1941289;Name=BBa_B0010
BBa_I20250	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1941284;Name=TetR 2
BBa_I20250	.	engineered_region	742	766	.	+	0	ID=annotation1941288;Name=barcode
BBa_I20250	.	promoter	43	48	.	+	0	ID=annotation1941285;Name=-10
BBa_I20250	.	engineered_region	863	903	.	+	0	ID=annotation1941291;Name=BBa_B0012
BBa_I20250	.	CDS	61	735	.	+	0	ID=annotation1941286;Name=mrfp1
BBa_I20250	.	stop_codon	896	896	.	+	0	ID=annotation1941294;Name=stop
BBa_I20250	.	engineered_region	61	741	.	+	0	ID=annotation1941287;Name=BBa_E1010
BBa_I20250	.	engineered_region	1	54	.	+	0	ID=annotation1941282;Name=BBa_R0040
BBa_I20250	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1941281;Name=TetR 1
BBa_I20250	.	polyA_site	890	903	.	+	0	ID=annotation1941293;Name=polya
BBa_I20250	.	stem_loop	870	889	.	+	0	ID=annotation1941292;Name=T7 TE
BBa_I20250	.	stem_loop	786	829	.	+	0	ID=annotation1941290;Name=stem_loop
BBa_I20250	.	promoter	20	25	.	+	0	ID=annotation1941283;Name=-35
>BBa_I20250
gaattgtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgaattcgcggccgctctag
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagt
tcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtcc
gctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatc
ccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccga
cggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa
ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaa
aaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgt
tgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataatgcag