##gff-version 3
##sequence-region BBa_I20243 1 3920
BBa_I20243	.	promoter	1	44	.	+	0	ID=BBa_R0184;Name=BBa_R0184
BBa_I20243	.	operator	20	44	.	+	0	ID=annotation1932679;Name=lacI binding site;Parent=BBa_R0184
BBa_I20243	.	sequence_feature	31	31	.	+	0	ID=annotation1932680;Name=Center of binding site symmetry;Parent=BBa_R0184
BBa_I20243	.	promoter	1	20	.	+	0	ID=annotation1932678;Name=BBa_R0183 fragment;Parent=BBa_R0184
BBa_I20243	.	engineered_region	53	3920	.	+	0	ID=BBa_I20241;Name=BBa_I20241
BBa_I20243	.	engineered_region	53	1203	.	+	0	ID=BBa_S03746;Name=BBa_S03746;Parent=BBa_I20241
BBa_I20243	.	ribosome_entry_site	1191	1203	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S03746
BBa_I20243	.	engineered_region	1191	1203	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I20243	.	ribosome_entry_site	1197	1200	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I20243	.	ribosome_entry_site	1191	1203	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I20243	.	engineered_region	53	1182	.	+	0	ID=BBa_S03690;Name=BBa_S03690;Parent=BBa_S03746
BBa_I20243	.	ribosome_entry_site	53	65	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S03690
BBa_I20243	.	engineered_region	53	65	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I20243	.	ribosome_entry_site	59	62	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I20243	.	ribosome_entry_site	53	65	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I20243	.	CDS	72	1182	.	+	0	ID=BBa_C0013;Name=BBa_C0013;Parent=BBa_S03690
BBa_I20243	.	engineered_tag	1158	1182	.	+	0	ID=annotation2214005;Name=Help:Barcodes;Parent=BBa_C0013
BBa_I20243	.	engineered_region	1210	3920	.	+	0	ID=BBa_S03693;Name=BBa_S03693;Parent=BBa_I20241
BBa_I20243	.	terminator	3873	3920	.	+	0	ID=BBa_B0016;Name=BBa_B0016;Parent=BBa_S03693
BBa_I20243	.	CDS	1210	3864	.	+	0	ID=BBa_I2032;Name=BBa_I2032;Parent=BBa_S03693
BBa_I20243	.	promoter	3707	3734	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
>BBa_I20243
tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacacaggaaagtactagatgaaacc
agtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac
gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaac
tggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggc
gattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaa
gcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattg
ctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatttt
ctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggc
ccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatag
cggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccac
tgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcg
gatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatt
ttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgtt
gcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattca
ttaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactac
tagagtcacacaggaaagtactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgcta
tcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacga
gatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaag
cctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggca
agcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggc
ttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgc
ttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacg
tctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttc
gtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagctta
caccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaa
cccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattac
tggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctac
gaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcc
tagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactccc
gatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaag
gacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggcca
tctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgac
caaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaac
tgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcg
ctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgc
tggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcac
ttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacg
ggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgac
cgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggt
gttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgc
tggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacat
ggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgct
aagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactc
ctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccg
cttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaacttt
gtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcac
tgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacac
atatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatg
ccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactaga
gctagcataaccccttggggcctctaaacgggtcttgaggggttttttg