##gff-version 3
##sequence-region BBa_I2022 1 3576
BBa_I2022	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I2022	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I2022	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I2022	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I2022	.	engineered_region	2660	3576	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_I2022	.	engineered_region	2678	3439	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_I2022	.	engineered_region	2678	3439	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I2022	.	stop_codon	3434	3439	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I2022	.	tag	3395	3433	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I2022	.	CDS	2678	3439	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I2022	.	sequence_alteration	2919	2919	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I2022	.	ribosome_entry_site	2660	2671	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_I2022	.	conserved_region	2664	2667	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2022	.	terminator	3536	3576	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_I2022	.	engineered_region	3536	3576	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2022	.	stem_loop	3543	3562	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2022	.	polyA_site	3563	3576	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2022	.	stop_codon	3569	3569	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2022	.	terminator	3448	3527	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_I2022	.	engineered_region	3448	3527	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2022	.	stem_loop	3459	3502	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2022	.	engineered_region	1219	2135	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_I2022	.	terminator	2095	2135	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_I2022	.	engineered_region	2095	2135	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2022	.	stem_loop	2102	2121	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2022	.	polyA_site	2122	2135	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2022	.	stop_codon	2128	2128	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2022	.	terminator	2007	2086	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_I2022	.	engineered_region	2007	2086	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2022	.	stem_loop	2018	2061	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2022	.	engineered_region	1237	1998	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_I2022	.	stop_codon	1993	1998	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I2022	.	CDS	1237	1998	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I2022	.	tag	1955	1992	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I2022	.	engineered_region	1237	1998	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I2022	.	point_mutation	1305	1305	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I2022	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_I2022	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2022	.	engineered_region	2144	2651	.	+	0	ID=BBa_I2012;Name=BBa_I2012
BBa_I2022	.	engineered_region	2162	2514	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_I2012
BBa_I2022	.	sequence_alteration	2239	2239	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_I2022	.	CDS	2162	2509	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_I2022	.	promoter	2334	2352	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_I2022	.	sequence_alteration	2269	2269	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_I2022	.	promoter	2187	2196	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_I2022	.	sequence_feature	2360	2509	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I2022	.	sequence_alteration	2248	2248	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_I2022	.	sequence_alteration	2272	2272	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_I2022	.	sequence_alteration	2242	2242	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_I2022	.	sequence_feature	2162	2176	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I2022	.	terminator	2523	2651	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I2012
BBa_I2022	.	terminator	2611	2651	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I2022	.	engineered_region	2611	2651	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2022	.	stem_loop	2618	2637	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2022	.	polyA_site	2638	2651	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2022	.	stop_codon	2644	2644	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2022	.	terminator	2523	2602	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I2022	.	engineered_region	2523	2602	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2022	.	stem_loop	2534	2577	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2022	.	ribosome_entry_site	2144	2155	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I2012
BBa_I2022	.	conserved_region	2148	2151	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I2022
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttca
ccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaggg
cgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg
accaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcca
tgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagtt
cgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag
ctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaaga
tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt
gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtc
ctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaa
actacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaa
agctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttat
cgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgt
cgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagc
tggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatggtgagc
aagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcg
tgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgt
gccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcac
gacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaaga
cccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacgg
caacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggc
atcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccc
ccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacga
gaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagagg
cctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata