##gff-version 3
##sequence-region BBa_I15032 1 2858
BBa_I15032	.	terminator	2730	2809	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15032	.	stem_loop	2741	2784	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15032	.	engineered_region	2730	2809	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15032	.	promoter	1861	1914	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I15032	.	non_covalent_binding_site	1861	1879	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I15032	.	non_covalent_binding_site	1886	1904	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I15032	.	engineered_region	1861	1914	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I15032	.	promoter	1880	1885	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I15032	.	promoter	1903	1908	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I15032	.	ribosome_entry_site	1923	1934	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15032	.	conserved_region	1927	1930	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15032	.	ribosome_entry_site	1055	1066	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15032	.	conserved_region	1059	1062	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15032	.	terminator	1812	1852	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15032	.	stop_codon	1845	1845	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15032	.	polyA_site	1839	1852	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15032	.	stem_loop	1819	1838	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15032	.	engineered_region	1812	1852	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15032	.	promoter	950	1046	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_I15032	.	promoter	1020	1025	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_I15032	.	non_covalent_binding_site	1006	1022	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_I15032	.	promoter	1007	1007	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_I15032	.	non_covalent_binding_site	955	974	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_I15032	.	non_covalent_binding_site	1030	1046	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_I15032	.	promoter	1043	1046	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_I15032	.	engineered_region	950	1046	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_I15032	.	promoter	996	1001	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_I15032	.	terminator	901	941	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15032	.	stop_codon	934	934	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15032	.	polyA_site	928	941	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15032	.	stem_loop	908	927	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15032	.	engineered_region	901	941	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15032	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15032	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15032	.	terminator	2818	2858	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15032	.	stop_codon	2851	2851	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15032	.	polyA_site	2845	2858	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15032	.	stem_loop	2825	2844	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15032	.	engineered_region	2818	2858	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15032	.	terminator	1724	1803	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15032	.	stem_loop	1735	1778	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15032	.	engineered_region	1724	1803	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15032	.	CDS	82	804	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I15032	.	CDS	1073	1690	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I15032	.	engineered_region	1073	1690	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I15032	.	tag	1652	1683	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I15032	.	CDS	1073	1651	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I15032	.	engineered_tag	1691	1715	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I15032	.	terminator	813	892	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15032	.	stem_loop	824	867	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15032	.	engineered_region	813	892	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15032	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I15032	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I15032	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I15032	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I15032	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I15032	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I15032	.	CDS	1941	2696	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I15032	.	engineered_region	1941	2696	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I15032	.	engineered_region	1941	1942	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I15032	.	engineered_tag	2697	2721	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I15032	.	point_mutation	2432	2432	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I15032	.	point_mutation	2114	2114	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I15032	.	CDS	1941	2690	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
>BBa_I15032
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcca
caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc
aagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccaca
tgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg
caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc
aaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagc
agaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca
gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaa
gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc
tgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactat
tttacctctggcggtgatatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttgg
caattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagt
tgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaat
gtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaac
agagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactc
tgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaaca
gtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatg
tattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaa
cgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagt
gatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaat
aattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgt
gaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaa
aatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcacc
aattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggt
cttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaag
acaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcg
aaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaa
ggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaa
tgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaa
aaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata