##gff-version 3
##sequence-region BBa_I15031 1 2900
BBa_I15031	.	terminator	2860	2900	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15031	.	stop_codon	2893	2893	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15031	.	polyA_site	2887	2900	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15031	.	stem_loop	2867	2886	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15031	.	engineered_region	2860	2900	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15031	.	promoter	1903	1956	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I15031	.	non_covalent_binding_site	1903	1921	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I15031	.	non_covalent_binding_site	1928	1946	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I15031	.	engineered_region	1903	1956	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I15031	.	promoter	1922	1927	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I15031	.	promoter	1945	1950	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I15031	.	terminator	1854	1894	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15031	.	stop_codon	1887	1887	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15031	.	polyA_site	1881	1894	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15031	.	stem_loop	1861	1880	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15031	.	engineered_region	1854	1894	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15031	.	ribosome_entry_site	1965	1976	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15031	.	conserved_region	1969	1972	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15031	.	CDS	1983	2738	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I15031	.	engineered_region	1983	2738	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I15031	.	engineered_region	1983	1984	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I15031	.	engineered_tag	2739	2763	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I15031	.	point_mutation	2474	2474	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I15031	.	point_mutation	2156	2156	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I15031	.	CDS	1983	2732	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I15031	.	terminator	943	983	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15031	.	stop_codon	976	976	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15031	.	polyA_site	970	983	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15031	.	stem_loop	950	969	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15031	.	engineered_region	943	983	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15031	.	ribosome_entry_site	1097	1108	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15031	.	conserved_region	1101	1104	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15031	.	promoter	992	1088	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_I15031	.	promoter	1062	1067	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_I15031	.	non_covalent_binding_site	1048	1064	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_I15031	.	promoter	1049	1049	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_I15031	.	non_covalent_binding_site	997	1016	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_I15031	.	non_covalent_binding_site	1072	1088	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_I15031	.	promoter	1085	1088	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_I15031	.	engineered_region	992	1088	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_I15031	.	promoter	1038	1043	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_I15031	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15031	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15031	.	terminator	1766	1845	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15031	.	stem_loop	1777	1820	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15031	.	engineered_region	1766	1845	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15031	.	CDS	1115	1732	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I15031	.	engineered_region	1115	1732	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I15031	.	tag	1694	1725	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I15031	.	CDS	1115	1693	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I15031	.	engineered_tag	1733	1757	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I15031	.	terminator	2772	2851	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15031	.	stem_loop	2783	2826	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15031	.	engineered_region	2772	2851	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15031	.	terminator	855	934	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15031	.	stem_loop	866	909	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15031	.	engineered_region	855	934	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15031	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_I15031	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_I15031	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_I15031	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_I15031	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_I15031	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_I15031	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_I15031	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_I15031	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_I15031	.	CDS	124	846	.	+	0	ID=BBa_E0030;Name=BBa_E0030
>BBa_I15031
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggt
ggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacct
tcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccga
aggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggc
gacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagt
acaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgcca
caacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctg
cccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgg
agttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaa
aatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggaga
aatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtct
tcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataac
tcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtg
attatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaag
tcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctata
tataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgta
ttaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcc
tattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaa
atactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgat
attaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattcta
tggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaa
atatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaat
aaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggcta
acaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtat
gaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgat
ttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggtt
gcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttc
taaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcacta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata