##gff-version 3
##sequence-region BBa_I15023 1 1888
BBa_I15023	.	terminator	1760	1839	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15023	.	engineered_region	1760	1839	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15023	.	stem_loop	1771	1814	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15023	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15023	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15023	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15023	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15023	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15023	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15023	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15023	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I15023	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I15023	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I15023	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I15023	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I15023	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I15023	.	CDS	81	803	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_I15023	.	ribosome_entry_site	1011	1022	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15023	.	conserved_region	1015	1018	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15023	.	CDS	1029	1751	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I15023	.	promoter	949	1002	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I15023	.	promoter	968	973	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I15023	.	promoter	991	996	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I15023	.	non_covalent_binding_site	949	967	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I15023	.	non_covalent_binding_site	974	992	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I15023	.	engineered_region	949	1002	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I15023	.	terminator	1848	1888	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15023	.	stem_loop	1855	1874	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15023	.	engineered_region	1848	1888	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15023	.	polyA_site	1875	1888	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15023	.	stop_codon	1881	1881	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15023	.	terminator	812	891	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15023	.	engineered_region	812	891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15023	.	stem_loop	823	866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_I15023
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacat
gaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagca
gaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata