##gff-version 3
##sequence-region BBa_I15004 1 2805
BBa_I15004	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I15004	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I15004	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15004	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15004	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15004	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15004	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I15004	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15004	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15004	.	engineered_region	1870	2805	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_I15004	.	CDS	1888	2643	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_I15004	.	engineered_tag	2644	2668	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I15004	.	CDS	1888	2637	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I15004	.	engineered_region	1888	2643	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I15004	.	point_mutation	2061	2061	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I15004	.	engineered_region	1888	1889	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I15004	.	point_mutation	2379	2379	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I15004	.	terminator	2765	2805	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_I15004	.	polyA_site	2792	2805	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15004	.	stop_codon	2798	2798	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15004	.	engineered_region	2765	2805	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15004	.	stem_loop	2772	2791	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15004	.	terminator	2677	2756	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_I15004	.	stem_loop	2688	2731	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15004	.	engineered_region	2677	2756	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15004	.	ribosome_entry_site	1870	1881	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_I15004	.	conserved_region	1874	1877	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15004	.	promoter	1808	1861	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I15004	.	promoter	1850	1855	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I15004	.	non_covalent_binding_site	1833	1851	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I15004	.	promoter	1827	1832	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I15004	.	engineered_region	1808	1861	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I15004	.	non_covalent_binding_site	1808	1826	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I15004	.	promoter	138	191	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I15004	.	operator	140	156	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I15004	.	promoter	157	162	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I15004	.	engineered_region	138	191	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I15004	.	operator	163	179	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I15004	.	promoter	180	185	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I15004	.	CDS	219	993	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I15004	.	engineered_tag	969	993	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I15004	.	tag	930	962	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I15004	.	CDS	222	929	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I15004	.	engineered_region	1002	1799	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_I15004	.	ribosome_entry_site	1002	1013	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_I15004	.	conserved_region	1006	1009	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15004	.	CDS	1020	1637	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_I15004	.	engineered_tag	1638	1662	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I15004	.	engineered_region	1020	1637	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I15004	.	CDS	1020	1598	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I15004	.	tag	1599	1630	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I15004	.	terminator	1759	1799	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_I15004	.	polyA_site	1786	1799	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15004	.	stop_codon	1792	1792	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15004	.	engineered_region	1759	1799	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15004	.	stem_loop	1766	1785	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15004	.	terminator	1671	1750	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_I15004	.	stem_loop	1682	1725	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15004	.	engineered_region	1671	1750	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15004	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15004	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I15004
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaatt
gacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaac
cattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatc
ccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaat
gcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatct
acgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggc
agggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagt
gattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaa
tgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttac
cttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaat
gagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaact
acgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgactat
aatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgttt
aagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatata
tttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaag
tgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggt
aaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgtta
gtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtca
tcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagttt
aaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagat
ctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcag
tgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaac
ataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttat
ctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatat
ttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagta
gattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatg
taattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaat
gcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaatt
gttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaa
aagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgt
cactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaaca
ggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata