##gff-version 3
##sequence-region BBa_I15001 1 2779
BBa_I15001	.	terminator	2739	2779	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15001	.	stem_loop	2746	2765	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15001	.	engineered_region	2739	2779	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15001	.	polyA_site	2766	2779	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15001	.	stop_codon	2772	2772	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15001	.	CDS	1862	2617	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I15001	.	engineered_tag	2618	2642	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I15001	.	engineered_region	1862	1863	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I15001	.	point_mutation	2353	2353	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I15001	.	CDS	1862	2611	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I15001	.	engineered_region	1862	2617	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I15001	.	point_mutation	2035	2035	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I15001	.	ribosome_entry_site	981	992	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15001	.	conserved_region	985	988	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15001	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15001	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15001	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15001	.	mature_transcript_region	138	972	.	+	0	ID=BBa_I1020;Name=BBa_I1020
BBa_I15001	.	non_covalent_binding_site	164	180	.	+	0	ID=annotation1831;Name=LacO-1;Parent=BBa_I1020
BBa_I15001	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation1830;Name=LacO-1 region;Parent=BBa_I1020
BBa_I15001	.	promoter	181	186	.	+	0	ID=annotation1826;Name=-10;Parent=BBa_I1020
BBa_I15001	.	tag	934	972	.	+	0	ID=annotation1828;Name=SsrA;Parent=BBa_I1020
BBa_I15001	.	stem_loop	193	222	.	+	0	ID=annotation1820;Name=stem_loop;Parent=BBa_I1020
BBa_I15001	.	CDS	220	972	.	+	0	ID=annotation1824;Name=cI lambda;Parent=BBa_I1020
BBa_I15001	.	sequence_feature	226	227	.	+	0	ID=annotation1835;Name=Homology to I1023;Parent=BBa_I1020
BBa_I15001	.	engineered_region	138	972	.	+	0	ID=annotation7046;Name=BBa_I1020;Parent=BBa_I1020
BBa_I15001	.	start_codon	220	222	.	+	0	ID=annotation1823;Name=start;Parent=BBa_I1020
BBa_I15001	.	sequence_alteration	195	195	.	+	0	ID=annotation1833;Name=HH117a mutation specific to I1020 and I1023;Parent=BBa_I1020
BBa_I15001	.	stop_codon	967	972	.	+	0	ID=annotation1829;Name=2;Parent=BBa_I1020
BBa_I15001	.	sequence_alteration	223	225	.	+	0	ID=annotation1834;Name=C added;Parent=BBa_I1020
BBa_I15001	.	ribosome_entry_site	210	215	.	+	0	ID=annotation1821;Name=rbs;Parent=BBa_I1020
BBa_I15001	.	start_codon	193	193	.	+	0	ID=annotation1832;Name=start;Parent=BBa_I1020
BBa_I15001	.	sequence_feature	193	227	.	+	0	ID=annotation1827;Name=Complementary to Antisense mRNA I1023;Parent=BBa_I1020
BBa_I15001	.	promoter	158	163	.	+	0	ID=annotation1825;Name=-35;Parent=BBa_I1020
BBa_I15001	.	CDS	999	1616	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I15001	.	engineered_tag	1617	1641	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I15001	.	tag	1578	1609	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I15001	.	engineered_region	999	1616	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I15001	.	CDS	999	1577	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I15001	.	terminator	2651	2730	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15001	.	engineered_region	2651	2730	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15001	.	stem_loop	2662	2705	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15001	.	ribosome_entry_site	1844	1855	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I15001	.	conserved_region	1848	1851	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I15001	.	promoter	1787	1835	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I15001	.	promoter	1821	1826	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I15001	.	promoter	1801	1806	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I15001	.	terminator	1650	1729	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15001	.	engineered_region	1650	1729	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15001	.	stem_loop	1661	1704	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15001	.	terminator	1738	1778	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15001	.	stem_loop	1745	1764	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15001	.	engineered_region	1738	1778	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15001	.	polyA_site	1765	1778	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15001	.	stop_codon	1771	1771	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15001	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15001	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15001	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15001	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15001	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_I15001
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataaatgtgagcggataacat
tgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatcagacaacaagatgtgcagtactaagaaa
aagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggct
tatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcatt
aaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaa
atctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttc
aggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagc
cagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgac
ggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagt
ttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatg
caatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaa
aactacgctttagtagcttaataatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattt
tttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggac
ttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactg
aaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttgg
tcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaat
aactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatg
taacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaat
tcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgct
gcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagttaaatttcctcttttcaggccggaataact
ccctataatgcgccaccatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaat
aattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgt
gaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaa
aatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcacc
aattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggt
cttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaag
acaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcg
aaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaa
ggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaa
tgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaa
aaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata