##gff-version 3
##sequence-region BBa_I15000 1 778
BBa_I15000	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15000	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15000	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15000	.	promoter	138	234	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_I15000	.	non_covalent_binding_site	194	210	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_I15000	.	promoter	208	213	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_I15000	.	promoter	195	195	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_I15000	.	non_covalent_binding_site	143	162	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_I15000	.	promoter	231	234	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_I15000	.	non_covalent_binding_site	218	234	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_I15000	.	engineered_region	138	234	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_I15000	.	promoter	184	189	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_I15000	.	terminator	738	778	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15000	.	polyA_site	765	778	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15000	.	engineered_region	738	778	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15000	.	stop_codon	771	771	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15000	.	stem_loop	745	764	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15000	.	ribosome_entry_site	243	257	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I15000	.	ribosome_entry_site	243	257	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I15000	.	ribosome_entry_site	250	254	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I15000	.	engineered_region	243	257	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I15000	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I15000	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I15000	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I15000	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I15000	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I15000	.	terminator	650	729	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I15000	.	stem_loop	661	704	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I15000	.	engineered_region	650	729	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I15000	.	engineered_region	264	616	.	+	0	ID=BBa_E0033;Name=BBa_E0033
BBa_I15000	.	promoter	289	298	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_I15000	.	sequence_alteration	344	344	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_I15000	.	sequence_feature	264	278	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I15000	.	sequence_alteration	374	374	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_I15000	.	sequence_feature	462	611	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I15000	.	sequence_alteration	350	350	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_I15000	.	CDS	264	611	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_I15000	.	promoter	436	454	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_I15000	.	sequence_alteration	341	341	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_I15000	.	sequence_alteration	371	371	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
>BBa_I15000
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtac
aggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatac
tagagattaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaag
ctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcg
ataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctg
gcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataacgctgatagtgctagt
gtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata