##gff-version 3
##sequence-region BBa_I14057 1 2825
BBa_I14057	.	CDS	1260	2009	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I14057	.	CDS	1263	1970	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I14057	.	tag	1971	2003	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I14057	.	engineered_tag	2010	2034	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I14057	.	ribosome_entry_site	1243	1253	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_I14057	.	engineered_region	1243	1253	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_I14057	.	ribosome_entry_site	1243	1253	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_I14057	.	ribosome_entry_site	1249	1252	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_I14057	.	engineered_region	2064	2825	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I14057	.	stop_codon	2820	2825	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I14057	.	sequence_alteration	2305	2305	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I14057	.	engineered_region	2064	2825	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I14057	.	CDS	2064	2825	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I14057	.	tag	2781	2819	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I14057	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I14057	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I14057	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I14057	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I14057	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I14057	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I14057	.	ribosome_entry_site	2043	2057	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I14057	.	ribosome_entry_site	2043	2057	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I14057	.	ribosome_entry_site	2050	2054	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I14057	.	engineered_region	2043	2057	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I14057	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I14057	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I14057	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I14057	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I14057	.	CDS	82	1209	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I14057	.	engineered_region	82	1209	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I14057	.	CDS	82	1209	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I14057	.	engineered_tag	1210	1234	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I14057	.	tag	1171	1209	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
>BBa_I14057
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgt
ggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccc
aaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgc
cgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaag
cggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctg
gatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacac
ccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagca
aatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgc
aatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctga
atgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtc
cgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtta
accaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcgg
tgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaa
aactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggactactagatgag
cacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaat
gaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggca
tcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaat
cgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttt
tctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaa
ccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaag
ctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttggg
ggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaa
tgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgc
aaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagattaaagaggag
aaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaa
acggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcac
caccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctacccc
gaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaagg
acgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcat
cgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcc
gacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgacc
actaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccct
gagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatg
gacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa