##gff-version 3 ##sequence-region BBa_I14056 1 2827 BBa_I14056 . ribosome_entry_site 63 77 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_I14056 . ribosome_entry_site 63 77 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_I14056 . ribosome_entry_site 70 74 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_I14056 . engineered_region 63 77 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_I14056 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_I14056 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_I14056 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_I14056 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_I14056 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_I14056 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_I14056 . ribosome_entry_site 1245 1255 . + 0 ID=BBa_B0033;Name=BBa_B0033 BBa_I14056 . engineered_region 1245 1255 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_I14056 . ribosome_entry_site 1245 1255 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_I14056 . ribosome_entry_site 1251 1254 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_I14056 . CDS 84 1211 . + 0 ID=BBa_C0012;Name=BBa_C0012 BBa_I14056 . CDS 84 1211 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_I14056 . engineered_region 84 1211 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_I14056 . engineered_tag 1212 1236 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_I14056 . tag 1173 1211 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_I14056 . CDS 1262 2011 . + 0 ID=BBa_C0051;Name=BBa_C0051 BBa_I14056 . engineered_tag 2012 2036 . + 0 ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051 BBa_I14056 . tag 1973 2005 . + 0 ID=annotation23335;Name=LVA;Parent=BBa_C0051 BBa_I14056 . CDS 1265 1972 . + 0 ID=annotation23334;Name=cI lambda;Parent=BBa_C0051 BBa_I14056 . ribosome_entry_site 2045 2059 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_I14056 . ribosome_entry_site 2045 2059 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_I14056 . ribosome_entry_site 2052 2056 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_I14056 . engineered_region 2045 2059 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_I14056 . engineered_region 2066 2827 . + 0 ID=BBa_E0032;Name=BBa_E0032 BBa_I14056 . stop_codon 2822 2827 . + 0 ID=annotation2159;Name=2;Parent=BBa_E0032 BBa_I14056 . sequence_alteration 2307 2307 . + 0 ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032 BBa_I14056 . engineered_region 2066 2827 . + 0 ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032 BBa_I14056 . CDS 2066 2827 . + 0 ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032 BBa_I14056 . tag 2783 2821 . + 0 ID=annotation2160;Name=SsrA;Parent=BBa_E0032 >BBa_I14056 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata ctagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgc gtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattc ccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgc gccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacga agcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgc tggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagac acccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccag caaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactc gcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgct gaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgag tccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgt taaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggc ggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctct ccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacg aaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggactactagatg agcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaa atgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatgg catcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttca atcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttt tttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcac aaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagcca agctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttg ggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtaccc aatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgct gcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagattaaagagg agaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgt aaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgc accaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctacc ccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaa ggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggc atcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatgg ccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccga ccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgcc ctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggca tggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa