##gff-version 3
##sequence-region BBa_I14055 1 2828
BBa_I14055	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I14055	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I14055	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I14055	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I14055	.	ribosome_entry_site	1243	1256	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_I14055	.	conserved_region	1249	1252	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I14055	.	ribosome_entry_site	2046	2060	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I14055	.	ribosome_entry_site	2046	2060	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I14055	.	ribosome_entry_site	2053	2057	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I14055	.	engineered_region	2046	2060	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I14055	.	engineered_region	2067	2828	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I14055	.	stop_codon	2823	2828	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I14055	.	sequence_alteration	2308	2308	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I14055	.	engineered_region	2067	2828	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I14055	.	CDS	2067	2828	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I14055	.	tag	2784	2822	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I14055	.	CDS	82	1209	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I14055	.	engineered_region	82	1209	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I14055	.	CDS	82	1209	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I14055	.	engineered_tag	1210	1234	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I14055	.	tag	1171	1209	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I14055	.	CDS	1263	2012	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I14055	.	CDS	1266	1973	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I14055	.	tag	1974	2006	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I14055	.	engineered_tag	2013	2037	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I14055	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I14055	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I14055	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I14055	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I14055	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I14055	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_I14055
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgt
ggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccc
aaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgc
cgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaag
cggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctg
gatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacac
ccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagca
aatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgc
aatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctga
atgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtc
cgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtta
accaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcgg
tgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaa
aactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaacctactagat
gagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaa
aatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatg
gcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttc
aatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgtt
ttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagca
caaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagcc
aagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagactt
gggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacc
caatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgc
tgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagattaaagag
gagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacg
taaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctg
caccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctac
cccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttca
aggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaaggg
catcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatg
gccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccg
accactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgc
cctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggc
atggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa