##gff-version 3
##sequence-region BBa_I14054 1 1703
BBa_I14054	.	engineered_region	942	1703	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I14054	.	tag	1660	1697	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I14054	.	CDS	942	1703	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I14054	.	engineered_region	942	1703	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I14054	.	point_mutation	1010	1010	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I14054	.	stop_codon	1698	1703	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I14054	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I14054	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I14054	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I14054	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I14054	.	CDS	228	887	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I14054	.	engineered_tag	888	912	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I14054	.	CDS	231	847	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I14054	.	tag	848	881	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I14054	.	ribosome_entry_site	921	935	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I14054	.	ribosome_entry_site	921	935	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I14054	.	ribosome_entry_site	928	932	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I14054	.	engineered_region	921	935	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I14054	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I14054	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I14054	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I14054	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I14054	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I14054	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I14054	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I14054	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
>BBa_I14054
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgtccagat
tagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaact
cgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcc
attgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacg
ctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaaca
gtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagc
gctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta
ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttatt
cggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactac
gctttagtagcttaataacactgatagtgctagtgtagatcactactagagattaaagaggagaaatactagatggtga
gcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcag
cgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgccc
gtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagc
acgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaa
gacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggac
ggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacg
gcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacac
ccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaac
gagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaga
ggcctgctgcaaacgacgaaaactacgctttagtagcttaataa