##gff-version 3
##sequence-region BBa_I14047 1 3118
BBa_I14047	.	CDS	105	1232	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I14047	.	engineered_tag	1233	1257	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I14047	.	CDS	105	1232	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I14047	.	tag	1194	1232	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I14047	.	engineered_region	105	1232	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I14047	.	ribosome_entry_site	2233	2247	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I14047	.	engineered_region	2233	2247	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I14047	.	ribosome_entry_site	2240	2244	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I14047	.	ribosome_entry_site	2233	2247	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I14047	.	terminator	3024	3064	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I14047	.	engineered_region	3024	3064	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I14047	.	stem_loop	3031	3050	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I14047	.	polyA_site	3051	3064	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I14047	.	stop_codon	3057	3057	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I14047	.	ribosome_entry_site	1266	1278	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I14047	.	ribosome_entry_site	1266	1278	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I14047	.	engineered_region	1266	1278	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I14047	.	ribosome_entry_site	1272	1275	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I14047	.	CDS	1285	2199	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_I14047	.	engineered_tag	2200	2224	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_I14047	.	CDS	1285	2160	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_I14047	.	tag	2161	2193	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_I14047	.	ribosome_entry_site	86	98	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I14047	.	ribosome_entry_site	86	98	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I14047	.	engineered_region	86	98	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I14047	.	ribosome_entry_site	92	95	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I14047	.	terminator	3073	3118	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I14047	.	stem_loop	3085	3107	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I14047	.	engineered_region	3073	3118	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I14047	.	engineered_region	2254	3015	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I14047	.	tag	2971	3009	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I14047	.	sequence_alteration	2495	2495	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I14047	.	engineered_region	2254	3015	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I14047	.	stop_codon	3010	3015	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I14047	.	CDS	2254	3015	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I14047	.	promoter	1	77	.	+	0	ID=BBa_R0074;Name=BBa_R0074
BBa_I14047	.	non_covalent_binding_site	31	53	.	+	0	ID=annotation319788;Name=dimer left half;Parent=BBa_R0074
BBa_I14047	.	operator	1	77	.	+	0	ID=annotation302713;Name=PenI;Parent=BBa_R0074
BBa_I14047	.	promoter	23	28	.	+	0	ID=annotation319211;Name=-35;Parent=BBa_R0074
BBa_I14047	.	promoter	46	51	.	+	0	ID=annotation319212;Name=-10;Parent=BBa_R0074
BBa_I14047	.	non_covalent_binding_site	55	76	.	+	0	ID=annotation319789;Name=dimer right half;Parent=BBa_R0074
>BBa_I14047
tcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttgta
ctagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtc
tcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcga
tggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccac
ctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtg
gtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtg
ggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatt
tcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcat
ctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctg
gctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttt
tcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggc
gcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagaca
gctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgct
gcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcg
cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa
gcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggt
ggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatatt
ctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgc
tgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcg
tccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcg
caccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctgg
cgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcg
ggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtt
tgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaac
gcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacga
tcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaa
gtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgct
agtgtagatcactactagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggt
gcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacc
tacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcg
gctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaagg
ctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac
accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtaca
actacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaa
catcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgccc
gacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagt
tcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgcttt
agtagcttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatat
aaaaagccagattattaatccggcttttttattattt