##gff-version 3
##sequence-region BBa_I14030 1 2830
BBa_I14030	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I14030	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I14030	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I14030	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I14030	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I14030	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I14030	.	CDS	1265	2014	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I14030	.	tag	1976	2008	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I14030	.	engineered_tag	2015	2039	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I14030	.	CDS	1268	1975	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I14030	.	engineered_region	2069	2830	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I14030	.	CDS	2069	2830	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I14030	.	tag	2786	2824	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I14030	.	stop_codon	2825	2830	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I14030	.	sequence_alteration	2310	2310	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I14030	.	engineered_region	2069	2830	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I14030	.	ribosome_entry_site	1245	1258	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_I14030	.	conserved_region	1251	1254	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I14030	.	CDS	84	1211	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I14030	.	engineered_tag	1212	1236	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I14030	.	engineered_region	84	1211	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I14030	.	tag	1173	1211	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I14030	.	CDS	84	1211	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I14030	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I14030	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I14030	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I14030	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I14030	.	ribosome_entry_site	2048	2062	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I14030	.	ribosome_entry_site	2048	2062	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I14030	.	ribosome_entry_site	2055	2059	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I14030	.	engineered_region	2048	2062	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
>BBa_I14030
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgc
gtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattc
ccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgc
gccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacga
agcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgc
tggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagac
acccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccag
caaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactc
gcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgct
gaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgag
tccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgt
taaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggc
ggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctct
ccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacg
aaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaacctactag
atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaaga
aaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaa
tggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccct
tcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctg
ttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaag
cacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaag
ccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagac
ttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagta
cccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggc
gctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagattaaag
aggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcga
cgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatc
tgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgct
accccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttctt
caaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaag
ggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatca
tggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgc
cgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtcc
gccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcg
gcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa