##gff-version 3
##sequence-region BBa_I13977 1 2083
##sequence-region BBa_I13212 1 1095
BBa_I13977	.	engineered_region	1	980	.	+	0	ID=BBa_I13976;Name=BBa_I13976
BBa_I13977	.	engineered_region	64	980	.	+	0	ID=BBa_E0434;Name=BBa_E0434;Parent=BBa_I13976
BBa_I13977	.	terminator	852	931	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0434
BBa_I13977	.	engineered_region	852	931	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13977	.	stem_loop	863	906	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13977	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0434
BBa_I13977	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13977	.	engineered_region	82	843	.	+	0	ID=BBa_E0034;Name=BBa_E0034;Parent=BBa_E0434
BBa_I13977	.	tag	799	837	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I13977	.	stop_codon	838	843	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I13977	.	terminator	940	980	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0434
BBa_I13977	.	polyA_site	967	980	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13977	.	engineered_region	940	980	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13977	.	stem_loop	947	966	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13977	.	stop_codon	973	973	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13977	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I13976
BBa_I13977	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13977	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13977	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13977	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13977	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13977	.	engineered_region	989	2083	.	+	0	ID=BBa_S3511;Name=BBa_S3511
BBa_I13977	.	promoter	989	1139	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_S3511
BBa_I13977	.	non_covalent_binding_site	989	1008	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I13977	.	promoter	1077	1082	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I13977	.	engineered_region	989	1139	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I13977	.	non_covalent_binding_site	1118	1137	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I13977	.	start_codon	1116	1116	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I13977	.	promoter	1103	1110	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I13977	.	terminator	1955	2034	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S3511
BBa_I13977	.	engineered_region	1955	2034	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13977	.	stem_loop	1966	2009	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13977	.	CDS	1166	1921	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S3511
BBa_I13977	.	engineered_region	1166	1921	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13977	.	engineered_region	1166	1167	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13977	.	CDS	1166	1915	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13977	.	point_mutation	1339	1339	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13977	.	point_mutation	1657	1657	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13977	.	engineered_tag	1922	1946	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13977	.	ribosome_entry_site	1148	1159	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S3511
BBa_I13977	.	conserved_region	1152	1155	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13977	.	terminator	2043	2083	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S3511
BBa_I13977	.	polyA_site	2070	2083	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13977	.	engineered_region	2043	2083	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13977	.	stem_loop	2050	2069	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13977	.	stop_codon	2076	2076	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13212	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13212	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13212	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13212	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I13212	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13212	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13212	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13212	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13212	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13212	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13212	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I13212	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I13212	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I13212	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I13212	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I13212	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I13212	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I13212	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13212	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13212	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13212	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13212	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13212	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13212	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13977
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcca
caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc
aagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccaca
tgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg
caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc
aaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagc
agaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca
gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaa
gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc
tgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgt
attcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatata
taacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccga
cgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgact
aaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctag
ataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaa
ctccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaa
gcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttg
cacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctct
agttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgttta
gcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatt
taaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattga
ttgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata
>BBa_I13212
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata