##gff-version 3
##sequence-region BBa_I13905 1 4325
BBa_I13905	.	promoter	983	1133	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I13905	.	promoter	1071	1076	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I13905	.	start_codon	1110	1110	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I13905	.	non_covalent_binding_site	1112	1131	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I13905	.	non_covalent_binding_site	983	1002	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I13905	.	engineered_region	983	1133	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I13905	.	promoter	1097	1104	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I13905	.	CDS	2104	3231	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I13905	.	engineered_region	2104	3231	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13905	.	tag	3193	3231	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13905	.	engineered_tag	3232	3256	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13905	.	CDS	2104	3231	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13905	.	terminator	846	925	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13905	.	engineered_region	846	925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13905	.	stem_loop	857	900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13905	.	ribosome_entry_site	1142	1153	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13905	.	conserved_region	1146	1149	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13905	.	terminator	1949	2028	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13905	.	engineered_region	1949	2028	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13905	.	stem_loop	1960	2003	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13905	.	ribosome_entry_site	2086	2097	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13905	.	conserved_region	2090	2093	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13905	.	terminator	934	974	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13905	.	stem_loop	941	960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13905	.	engineered_region	934	974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13905	.	polyA_site	961	974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13905	.	stop_codon	967	967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13905	.	terminator	4134	4213	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13905	.	engineered_region	4134	4213	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13905	.	stem_loop	4145	4188	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13905	.	CDS	3483	4100	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I13905	.	engineered_tag	4101	4125	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I13905	.	tag	4062	4093	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I13905	.	engineered_region	3483	4100	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I13905	.	CDS	3483	4061	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I13905	.	terminator	4222	4262	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13905	.	stem_loop	4229	4248	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13905	.	engineered_region	4222	4262	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13905	.	polyA_site	4249	4262	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13905	.	stop_codon	4255	4255	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13905	.	promoter	3402	3455	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I13905	.	engineered_region	3402	3455	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13905	.	operator	3404	3420	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13905	.	operator	3427	3443	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13905	.	promoter	3444	3449	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13905	.	promoter	3421	3426	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13905	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I13905	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I13905	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I13905	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I13905	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I13905	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I13905	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13905	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13905	.	CDS	1160	1915	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I13905	.	engineered_tag	1916	1940	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13905	.	engineered_region	1160	1161	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13905	.	point_mutation	1651	1651	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13905	.	CDS	1160	1909	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13905	.	engineered_region	1160	1915	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13905	.	point_mutation	1333	1333	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13905	.	ribosome_entry_site	3465	3476	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13905	.	conserved_region	3469	3472	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13905	.	terminator	2037	2077	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13905	.	stem_loop	2044	2063	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13905	.	engineered_region	2037	2077	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13905	.	polyA_site	2064	2077	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13905	.	stop_codon	2070	2070	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13905	.	engineered_region	76	837	.	+	0	ID=BBa_E0034;Name=BBa_E0034
BBa_I13905	.	stop_codon	832	837	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I13905	.	tag	793	831	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I13905	.	terminator	3265	3344	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13905	.	engineered_region	3265	3344	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13905	.	stem_loop	3276	3319	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13905	.	terminator	3353	3393	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13905	.	stem_loop	3360	3379	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13905	.	engineered_region	3353	3393	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13905	.	polyA_site	3380	3393	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13905	.	stop_codon	3386	3386	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13905	.	promoter	4271	4325	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I13905	.	promoter	4290	4295	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13905	.	engineered_region	4271	4325	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13905	.	promoter	4312	4317	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13905	.	start_codon	4323	4323	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13905	.	non_covalent_binding_site	4271	4290	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_I13905
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagc
tgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaaga
acggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattccc
agcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacac
gcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacac
atacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatg
gtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataatt
accctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaa
tcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaa
acatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacatt
cagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttga
taattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgg
gcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaacca
atgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgccc
atactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatac
gatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaa
cgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaaca
gtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgc
gccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcaca
atcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgc
ctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagac
ggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctg
tctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaagg
cgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggtt
gccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtag
tgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggg
gcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactg
gtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctgg
cacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgata
gtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
tactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttc
gttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactc
aaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgat
tatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtc
gttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatata
taaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtatt
aaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgccta
ttaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgata
gtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa