##gff-version 3
##sequence-region BBa_I13904 1 3856
BBa_I13904	.	engineered_region	76	837	.	+	0	ID=BBa_E0034;Name=BBa_E0034
BBa_I13904	.	stop_codon	832	837	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I13904	.	tag	793	831	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I13904	.	promoter	983	1133	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I13904	.	start_codon	1110	1110	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I13904	.	engineered_region	983	1133	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I13904	.	non_covalent_binding_site	1112	1131	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I13904	.	non_covalent_binding_site	983	1002	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I13904	.	promoter	1097	1104	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I13904	.	promoter	1071	1076	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I13904	.	terminator	934	974	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13904	.	engineered_region	934	974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13904	.	polyA_site	961	974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13904	.	stop_codon	967	967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13904	.	stem_loop	941	960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13904	.	ribosome_entry_site	1142	1153	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13904	.	conserved_region	1146	1149	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13904	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13904	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13904	.	terminator	3816	3856	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13904	.	engineered_region	3816	3856	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13904	.	polyA_site	3843	3856	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13904	.	stop_codon	3849	3849	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13904	.	stem_loop	3823	3842	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13904	.	ribosome_entry_site	3059	3070	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13904	.	conserved_region	3063	3066	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13904	.	CDS	2167	2826	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I13904	.	tag	2787	2820	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13904	.	engineered_tag	2827	2851	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13904	.	CDS	2170	2786	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13904	.	terminator	1949	2028	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13904	.	engineered_region	1949	2028	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13904	.	stem_loop	1960	2003	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13904	.	terminator	2948	2988	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13904	.	engineered_region	2948	2988	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13904	.	polyA_site	2975	2988	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13904	.	stop_codon	2981	2981	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13904	.	stem_loop	2955	2974	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13904	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I13904	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I13904	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I13904	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I13904	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I13904	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I13904	.	terminator	2860	2939	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13904	.	engineered_region	2860	2939	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13904	.	stem_loop	2871	2914	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13904	.	promoter	2997	3050	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13904	.	non_covalent_binding_site	3022	3040	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13904	.	promoter	3039	3044	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13904	.	non_covalent_binding_site	2997	3015	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13904	.	engineered_region	2997	3050	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13904	.	promoter	3016	3021	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13904	.	terminator	2037	2077	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13904	.	engineered_region	2037	2077	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13904	.	polyA_site	2064	2077	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13904	.	stop_codon	2070	2070	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13904	.	stem_loop	2044	2063	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13904	.	terminator	3728	3807	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13904	.	engineered_region	3728	3807	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13904	.	stem_loop	3739	3782	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13904	.	CDS	3077	3694	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I13904	.	engineered_region	3077	3694	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I13904	.	engineered_tag	3695	3719	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I13904	.	tag	3656	3687	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I13904	.	CDS	3077	3655	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I13904	.	CDS	1160	1915	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I13904	.	engineered_tag	1916	1940	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13904	.	point_mutation	1651	1651	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13904	.	engineered_region	1160	1161	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13904	.	point_mutation	1333	1333	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13904	.	engineered_region	1160	1915	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13904	.	CDS	1160	1909	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13904	.	terminator	846	925	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13904	.	engineered_region	846	925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13904	.	stem_loop	857	900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13904	.	ribosome_entry_site	2149	2160	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13904	.	conserved_region	2153	2156	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13904	.	promoter	2086	2140	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I13904	.	non_covalent_binding_site	2086	2105	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13904	.	promoter	2105	2110	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13904	.	engineered_region	2086	2140	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13904	.	start_codon	2138	2138	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13904	.	promoter	2127	2132	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
>BBa_I13904
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagc
tgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaaga
acggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattccc
agcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacac
gcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacac
atacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatg
gtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataatt
accctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaa
tcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaa
acatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacatt
cagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttga
taattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgg
gcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaacca
atgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgccc
atactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtc
gaataaatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagc
tgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgta
ttggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgc
cctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcg
atggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatg
ccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaa
gatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagcta
tcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaaca
acttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccta
tcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgac
tataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtg
tttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaat
atatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaa
aagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgta
ggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctg
ttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttg
tcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacag
tttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgta
gatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata