##gff-version 3
##sequence-region BBa_I13730 1 2410
BBa_I13730	.	engineered_region	1	1296	.	+	0	ID=BBa_I13721;Name=BBa_I13721
BBa_I13730	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I13721
BBa_I13730	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13730	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13730	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13730	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13730	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13730	.	terminator	1168	1296	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I13721
BBa_I13730	.	terminator	1256	1296	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13730	.	engineered_region	1256	1296	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13730	.	polyA_site	1283	1296	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13730	.	stem_loop	1263	1282	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13730	.	stop_codon	1289	1289	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13730	.	terminator	1168	1247	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13730	.	engineered_region	1168	1247	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13730	.	stem_loop	1179	1222	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13730	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13721
BBa_I13730	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13730	.	CDS	82	1159	.	+	0	ID=BBa_C0024;Name=BBa_C0024;Parent=BBa_I13721
BBa_I13730	.	CDS	82	1134	.	+	0	ID=annotation1891614;Name=CheB;Parent=BBa_C0024
BBa_I13730	.	engineered_tag	1135	1159	.	+	0	ID=annotation2214017;Name=Help:Barcodes;Parent=BBa_C0024
BBa_I13730	.	engineered_region	1305	2410	.	+	0	ID=BBa_I13712;Name=BBa_I13712
BBa_I13730	.	terminator	2282	2361	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13712
BBa_I13730	.	engineered_region	2282	2361	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13730	.	stem_loop	2293	2336	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13730	.	promoter	1305	1358	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13712
BBa_I13730	.	engineered_region	1305	1358	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13730	.	promoter	1347	1352	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13730	.	non_covalent_binding_site	1330	1348	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13730	.	non_covalent_binding_site	1305	1323	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13730	.	promoter	1324	1329	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13730	.	terminator	2370	2410	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13712
BBa_I13730	.	engineered_region	2370	2410	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13730	.	polyA_site	2397	2410	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13730	.	stem_loop	2377	2396	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13730	.	stop_codon	2403	2403	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13730	.	CDS	1385	2248	.	+	0	ID=BBa_C0028;Name=BBa_C0028;Parent=BBa_I13712
BBa_I13730	.	engineered_tag	2249	2273	.	+	0	ID=annotation2214018;Name=Help:Barcodes;Parent=BBa_C0028
BBa_I13730	.	CDS	1385	2248	.	+	0	ID=annotation1891584;Name=CheB;Parent=BBa_C0028
BBa_I13730	.	ribosome_entry_site	1367	1378	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13712
BBa_I13730	.	conserved_region	1371	1374	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13730
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagcaaaatcagggtgttatctgtcgatgattcggcactgatgcgccagatcatgacagaaatcatcaacagcca
tagcgacatggaaatggtggcgaccgcgcctgatccgctggtcgcgcgtgacttgattaagaaattcaatcccgatgtg
ctgacgctggatgttgaaatgccgcggatggacggactggatttcctcgaaaaattaatgcgtttgcgtccaatgcccg
ttgtgatggtttcttccctgaccggcaaagggtcagaagtcacgctgcgcgcgctggagctgggggcgatagattttgt
caccaaaccgcaactgggtattcgcgaaggtatgctggcgtataacgaaatgattgctgaaaaggtgcgtacggcagca
aaggcgagccttgcagcacataagccattgtcggcaccgacaacgctgaaggcggggccgttgttgagttctgaaaaac
tgattgcgattggtgcttcaacgggtggaactgaggcaattcgtcacgtactgcaaccgttgccgctttccagcccggc
actgttaattacccagcatatgccgcccggtttcacccgctcttttgccgacagacttaataagctttgccagatcggg
gttaaagaagccgaagacggagaacgtgtcttgccggggcatgcctatattgcgccgggcgatcggcatatggagctgt
cgcgtagtggcgcaaattaccaaatcaaaattcacgatggcccggcggttaaccgtcatcggccttcggtagatgtgtt
gttccattctgtcgccaaacaggcggggcgtaatgcggttggggtgatcctgaccggtatgggcaacgacggcgcggcg
ggaatgttggcgatgcgtcaggcgggggcatggacccttgcgcaaaacgaagcaagttgcgtggtgttcggcatgccgc
gcgaggccatcaatatgggtggtgtctgcgaagtggtcgatcttagccaggtaagccagcaaatgttggcaaaaattag
tgccggacaggcgatacgtatttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgat
agagatactgagcactactagagaaagaggagaaatactagatgacttcatctctgccctgtgggcaaacgtctttatt
gttacagatgaccgagcgcctggcgctttccgacgcgcattttcggcggataagtcaattgatctatcaacgagccggg
atcgttctggctgaccataaacgcgacatggtttacaaccgactggttcgtcgtttgcgttcgctgggactgacggatt
tcggtcattatctgaacttgctggaatctaatcagcacagcggtgagtggcaggcgtttatcaattcgctgaccacgaa
tctgacggcatttttccgtgaggcacatcatttccctctgctcgcggatcacgcacgtcgccgttctggcgagtatcgc
gtatggagcgcggcggcttcgaccggcgaagagccgtacagcattgcgatgacgctggctgacacattgggcaccgcgc
ccggacgctggaaagtgtttgccagtgatatcgacaccgaagtgctggaaaaagccagaagcggtatctatcgccatga
agagttgaaaaacctgacgccgcagcaactgcaacggtatttcatgcgagggacggggccgcatgaagggctggtacgc
gtgcgtcaggagctggcgaactatgttgattttgccccgctgaatctactggcgaaacagtacaccgtgccggggccgt
ttgatgcgatcttctgtcgtaacgtcatgatctacttcgatcaaactacccagcaggagattttgcgccgctttgttcc
gctccttaaacccgacggattgctgtttgcgggtcactctgaaaactttagccaccttgagcgccgcttcacgctgcgt
ggtcagacggtgtatgcgctaagtaaggattaataacgctgatagtgctagtgtagatcgctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata