##gff-version 3
##sequence-region BBa_I13712 1 1106
BBa_I13712	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13712	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13712	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13712	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13712	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13712	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13712	.	terminator	978	1057	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13712	.	stem_loop	989	1032	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13712	.	engineered_region	978	1057	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13712	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13712	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13712	.	CDS	81	944	.	+	0	ID=BBa_C0028;Name=BBa_C0028
BBa_I13712	.	CDS	81	944	.	+	0	ID=annotation1891584;Name=CheB;Parent=BBa_C0028
BBa_I13712	.	engineered_tag	945	969	.	+	0	ID=annotation2214018;Name=Help:Barcodes;Parent=BBa_C0028
BBa_I13712	.	terminator	1066	1106	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13712	.	polyA_site	1093	1106	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13712	.	engineered_region	1066	1106	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13712	.	stop_codon	1099	1099	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13712	.	stem_loop	1073	1092	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I13712
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgacttcatctctgccctgtgggcaaacgtctttattgttacagatgaccgagcgcctggcgctttccgacgcgcat
tttcggcggataagtcaattgatctatcaacgagccgggatcgttctggctgaccataaacgcgacatggtttacaacc
gactggttcgtcgtttgcgttcgctgggactgacggatttcggtcattatctgaacttgctggaatctaatcagcacag
cggtgagtggcaggcgtttatcaattcgctgaccacgaatctgacggcatttttccgtgaggcacatcatttccctctg
ctcgcggatcacgcacgtcgccgttctggcgagtatcgcgtatggagcgcggcggcttcgaccggcgaagagccgtaca
gcattgcgatgacgctggctgacacattgggcaccgcgcccggacgctggaaagtgtttgccagtgatatcgacaccga
agtgctggaaaaagccagaagcggtatctatcgccatgaagagttgaaaaacctgacgccgcagcaactgcaacggtat
ttcatgcgagggacggggccgcatgaagggctggtacgcgtgcgtcaggagctggcgaactatgttgattttgccccgc
tgaatctactggcgaaacagtacaccgtgccggggccgtttgatgcgatcttctgtcgtaacgtcatgatctacttcga
tcaaactacccagcaggagattttgcgccgctttgttccgctccttaaacccgacggattgctgtttgcgggtcactct
gaaaactttagccaccttgagcgccgcttcacgctgcgtggtcagacggtgtatgcgctaagtaaggattaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata