##gff-version 3
##sequence-region BBa_I13665 1 2340
BBa_I13665	.	engineered_region	58	1430	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13665	.	promoter	1376	1429	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13665	.	promoter	1418	1423	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13665	.	operator	1401	1417	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13665	.	operator	1378	1394	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13665	.	engineered_region	1376	1429	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13665	.	promoter	1395	1400	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13665	.	ribosome_entry_site	58	71	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13665	.	conserved_region	64	67	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13665	.	CDS	78	1205	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13665	.	engineered_tag	1206	1230	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13665	.	CDS	78	1205	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13665	.	tag	1167	1205	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13665	.	engineered_region	78	1205	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13665	.	terminator	1239	1367	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13665	.	terminator	1239	1318	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13665	.	stem_loop	1250	1293	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13665	.	engineered_region	1239	1318	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13665	.	terminator	1327	1367	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13665	.	engineered_region	1327	1367	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13665	.	stem_loop	1334	1353	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13665	.	stop_codon	1360	1360	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13665	.	polyA_site	1354	1367	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13665	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I13665	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I13665	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I13665	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I13665	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I13665	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I13665	.	engineered_region	1439	2340	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I13665	.	ribosome_entry_site	1439	1450	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I13665	.	conserved_region	1443	1446	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13665	.	promoter	2287	2340	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I13665	.	promoter	2306	2311	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13665	.	non_covalent_binding_site	2312	2330	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13665	.	engineered_region	2287	2340	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13665	.	non_covalent_binding_site	2287	2305	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13665	.	promoter	2329	2334	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13665	.	terminator	2238	2278	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I13665	.	engineered_region	2238	2278	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13665	.	stem_loop	2245	2264	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13665	.	stop_codon	2271	2271	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13665	.	polyA_site	2265	2278	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13665	.	CDS	1457	2116	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I13665	.	engineered_tag	2117	2141	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13665	.	CDS	1460	2076	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13665	.	tag	2077	2110	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13665	.	terminator	2150	2229	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I13665	.	stem_loop	2161	2204	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13665	.	engineered_region	2150	2229	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I13665
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaacctactagat
ggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg
aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaacc
gcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtc
gcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggc
gtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatg
accaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccat
caacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatc
gcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc
aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatga
gggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccggg
ctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacca
ccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaa
gggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc
gcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaact
acgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatac
tgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagag
ctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgt
attggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttg
ccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgc
gatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttat
gccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattgga
agatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagct
atcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaac
aacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtg
tagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccct
atcagtgatagagattgacatccctatcagtgatagagatactgagcac