##gff-version 3
##sequence-region BBa_I13657 1 2340
BBa_I13657	.	engineered_region	968	2340	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13657	.	terminator	2149	2277	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13657	.	terminator	2149	2228	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13657	.	stem_loop	2160	2203	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13657	.	engineered_region	2149	2228	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13657	.	terminator	2237	2277	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13657	.	stop_codon	2270	2270	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13657	.	polyA_site	2264	2277	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13657	.	stem_loop	2244	2263	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13657	.	engineered_region	2237	2277	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13657	.	promoter	2286	2339	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13657	.	promoter	2328	2333	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13657	.	operator	2288	2304	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13657	.	promoter	2305	2310	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13657	.	engineered_region	2286	2339	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13657	.	operator	2311	2327	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13657	.	ribosome_entry_site	968	981	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13657	.	conserved_region	974	977	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13657	.	CDS	988	2115	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13657	.	engineered_region	988	2115	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13657	.	tag	2077	2115	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13657	.	CDS	988	2115	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13657	.	engineered_tag	2116	2140	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13657	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I13657	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I13657	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I13657	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I13657	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I13657	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I13657	.	engineered_region	58	959	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I13657	.	terminator	857	897	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I13657	.	stop_codon	890	890	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13657	.	polyA_site	884	897	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13657	.	stem_loop	864	883	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13657	.	engineered_region	857	897	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13657	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I13657	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13657	.	promoter	906	959	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I13657	.	non_covalent_binding_site	906	924	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13657	.	non_covalent_binding_site	931	949	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13657	.	engineered_region	906	959	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13657	.	promoter	925	930	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13657	.	promoter	948	953	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13657	.	CDS	76	735	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I13657	.	tag	696	729	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13657	.	engineered_tag	736	760	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13657	.	CDS	79	695	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13657	.	terminator	769	848	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I13657	.	stem_loop	780	823	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13657	.	engineered_region	769	848	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I13657
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgt
ccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccg
taaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcc
ttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgta
ataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacaga
aaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgca
ctcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaa
cacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcctt
cttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaa
aactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagag
atactgagcactactagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcag
agtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaa
agtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctg
attggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaac
tgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgc
gcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaat
gttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgac
tgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcg
tctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagt
gccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatc
agatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacga
cgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagc
gtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa
aaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgt
gagcggataacaattgacattgtgagcggataacaagatactgagcaca