##gff-version 3
##sequence-region BBa_I13654 1 2342
BBa_I13654	.	engineered_region	970	2342	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13654	.	promoter	2288	2341	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13654	.	operator	2313	2329	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13654	.	operator	2290	2306	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13654	.	promoter	2330	2335	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13654	.	engineered_region	2288	2341	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13654	.	promoter	2307	2312	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13654	.	terminator	2151	2279	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13654	.	terminator	2239	2279	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13654	.	engineered_region	2239	2279	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13654	.	polyA_site	2266	2279	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13654	.	stop_codon	2272	2272	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13654	.	stem_loop	2246	2265	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13654	.	terminator	2151	2230	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13654	.	engineered_region	2151	2230	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13654	.	stem_loop	2162	2205	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13654	.	ribosome_entry_site	970	983	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13654	.	conserved_region	976	979	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13654	.	CDS	990	2117	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13654	.	engineered_region	990	2117	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13654	.	engineered_tag	2118	2142	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13654	.	tag	2079	2117	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13654	.	CDS	990	2117	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13654	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I13654	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I13654	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I13654	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I13654	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I13654	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I13654	.	engineered_region	58	961	.	+	0	ID=BBa_Q01400;Name=BBa_Q01400
BBa_I13654	.	ribosome_entry_site	58	71	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01400
BBa_I13654	.	conserved_region	64	67	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13654	.	promoter	908	961	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q01400
BBa_I13654	.	non_covalent_binding_site	933	951	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13654	.	promoter	950	955	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13654	.	non_covalent_binding_site	908	926	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13654	.	engineered_region	908	961	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13654	.	promoter	927	932	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13654	.	CDS	78	737	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q01400
BBa_I13654	.	engineered_tag	738	762	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13654	.	CDS	81	697	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13654	.	tag	698	731	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13654	.	terminator	771	850	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01400
BBa_I13654	.	engineered_region	771	850	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13654	.	stem_loop	782	825	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13654	.	terminator	859	899	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01400
BBa_I13654	.	engineered_region	859	899	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13654	.	polyA_site	886	899	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13654	.	stop_codon	892	892	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13654	.	stem_loop	866	885	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I13654
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaacctactagat
gtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacc
cgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacg
ccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacg
taataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctaca
gaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatg
cactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggga
aacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcc
ttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatag
agatactgagcactactagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgc
agagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaa
aaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgc
tgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatca
actgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctc
gcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcacta
atgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcg
actgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcg
cgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactgga
gtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacga
tcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatac
gacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaacca
gcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaag
aaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacag
gtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagt
gtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaatt
gtgagcggataacaattgacattgtgagcggataacaagatactgagcaca