##gff-version 3
##sequence-region BBa_I13645 1 2420
BBa_I13645	.	engineered_region	1519	2420	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I13645	.	ribosome_entry_site	1519	1530	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I13645	.	conserved_region	1523	1526	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13645	.	CDS	1537	2196	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I13645	.	tag	2157	2190	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13645	.	engineered_tag	2197	2221	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13645	.	CDS	1540	2156	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13645	.	promoter	2367	2420	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I13645	.	promoter	2409	2414	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13645	.	promoter	2386	2391	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13645	.	non_covalent_binding_site	2392	2410	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13645	.	engineered_region	2367	2420	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13645	.	non_covalent_binding_site	2367	2385	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13645	.	terminator	2318	2358	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I13645	.	engineered_region	2318	2358	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13645	.	stop_codon	2351	2351	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13645	.	polyA_site	2345	2358	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13645	.	stem_loop	2325	2344	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13645	.	terminator	2230	2309	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I13645	.	stem_loop	2241	2284	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13645	.	engineered_region	2230	2309	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13645	.	engineered_region	138	1510	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13645	.	CDS	158	1285	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13645	.	engineered_tag	1286	1310	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13645	.	CDS	158	1285	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13645	.	tag	1247	1285	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13645	.	engineered_region	158	1285	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13645	.	promoter	1456	1509	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13645	.	promoter	1475	1480	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13645	.	promoter	1498	1503	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13645	.	operator	1458	1474	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13645	.	engineered_region	1456	1509	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13645	.	operator	1481	1497	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13645	.	ribosome_entry_site	138	151	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13645	.	conserved_region	144	147	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13645	.	terminator	1319	1447	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13645	.	terminator	1319	1398	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13645	.	stem_loop	1330	1373	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13645	.	engineered_region	1319	1398	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13645	.	terminator	1407	1447	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13645	.	engineered_region	1407	1447	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13645	.	stop_codon	1440	1440	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13645	.	polyA_site	1434	1447	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13645	.	stem_loop	1414	1433	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13645	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I13645	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13645	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13645	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13645	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13645	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13645	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13645	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13645	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I13645
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacacaggaaacctactaga
tggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggt
gaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaac
cgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgt
cgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcgg
cgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggat
gaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacaccca
tcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaat
cgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaat
caaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatg
agggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgg
gctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacc
accatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtga
agggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccg
cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaac
tacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagata
ctgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattaga
gctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattg
tattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactttt
gccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcg
cgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttttta
tgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattgg
aagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagc
tatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaa
caacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccc
tatcagtgatagagattgacatccctatcagtgatagagatactgagcac