##gff-version 3
##sequence-region BBa_I13644 1 2422
BBa_I13644	.	engineered_region	138	1510	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13644	.	ribosome_entry_site	138	151	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13644	.	conserved_region	144	147	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13644	.	promoter	1456	1509	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13644	.	operator	1458	1474	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13644	.	engineered_region	1456	1509	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13644	.	promoter	1475	1480	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13644	.	operator	1481	1497	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13644	.	promoter	1498	1503	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13644	.	terminator	1319	1447	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13644	.	terminator	1319	1398	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13644	.	stem_loop	1330	1373	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13644	.	engineered_region	1319	1398	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13644	.	terminator	1407	1447	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13644	.	polyA_site	1434	1447	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13644	.	stem_loop	1414	1433	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13644	.	engineered_region	1407	1447	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13644	.	stop_codon	1440	1440	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13644	.	CDS	158	1285	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13644	.	engineered_region	158	1285	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13644	.	CDS	158	1285	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13644	.	engineered_tag	1286	1310	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13644	.	tag	1247	1285	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13644	.	engineered_region	1519	2422	.	+	0	ID=BBa_Q01400;Name=BBa_Q01400
BBa_I13644	.	terminator	2232	2311	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01400
BBa_I13644	.	stem_loop	2243	2286	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13644	.	engineered_region	2232	2311	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13644	.	promoter	2369	2422	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q01400
BBa_I13644	.	promoter	2388	2393	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13644	.	promoter	2411	2416	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13644	.	non_covalent_binding_site	2369	2387	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13644	.	engineered_region	2369	2422	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13644	.	non_covalent_binding_site	2394	2412	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13644	.	CDS	1539	2198	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q01400
BBa_I13644	.	CDS	1542	2158	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13644	.	tag	2159	2192	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13644	.	engineered_tag	2199	2223	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13644	.	terminator	2320	2360	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01400
BBa_I13644	.	polyA_site	2347	2360	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13644	.	stem_loop	2327	2346	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13644	.	engineered_region	2320	2360	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13644	.	stop_codon	2353	2353	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13644	.	ribosome_entry_site	1519	1532	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01400
BBa_I13644	.	conserved_region	1525	1528	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13644	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I13644	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13644	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13644	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13644	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13644	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13644	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13644	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13644	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_I13644
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacacaggaaacctactaga
tggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggt
gaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaac
cgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgt
cgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcgg
cgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggat
gaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacaccca
tcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaat
cgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaat
caaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatg
agggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgg
gctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacc
accatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtga
agggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccg
cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaac
tacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagata
ctgagcacatactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcatta
gagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacat
tgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactt
ttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcat
cgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttt
tatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtatt
ggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaa
gctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaa
aacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtc
cctatcagtgatagagattgacatccctatcagtgatagagatactgagcac