##gff-version 3
##sequence-region BBa_I13637 1 2420
BBa_I13637	.	engineered_region	138	1039	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I13637	.	promoter	986	1039	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I13637	.	non_covalent_binding_site	1011	1029	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13637	.	engineered_region	986	1039	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13637	.	promoter	1005	1010	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13637	.	non_covalent_binding_site	986	1004	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13637	.	promoter	1028	1033	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13637	.	CDS	156	815	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I13637	.	tag	776	809	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13637	.	engineered_tag	816	840	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13637	.	CDS	159	775	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13637	.	ribosome_entry_site	138	149	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I13637	.	conserved_region	142	145	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13637	.	terminator	937	977	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I13637	.	engineered_region	937	977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13637	.	polyA_site	964	977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13637	.	stop_codon	970	970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13637	.	stem_loop	944	963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13637	.	terminator	849	928	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I13637	.	engineered_region	849	928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13637	.	stem_loop	860	903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13637	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I13637	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13637	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13637	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13637	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13637	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13637	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13637	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13637	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13637	.	engineered_region	1048	2420	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13637	.	ribosome_entry_site	1048	1061	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13637	.	conserved_region	1054	1057	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13637	.	promoter	2366	2419	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13637	.	promoter	2385	2390	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13637	.	operator	2368	2384	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13637	.	promoter	2408	2413	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13637	.	engineered_region	2366	2419	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13637	.	operator	2391	2407	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13637	.	CDS	1068	2195	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13637	.	engineered_tag	2196	2220	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13637	.	engineered_region	1068	2195	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13637	.	CDS	1068	2195	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13637	.	tag	2157	2195	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13637	.	terminator	2229	2357	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13637	.	terminator	2317	2357	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13637	.	engineered_region	2317	2357	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13637	.	polyA_site	2344	2357	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13637	.	stop_codon	2350	2350	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13637	.	stem_loop	2324	2343	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13637	.	terminator	2229	2308	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13637	.	engineered_region	2229	2308	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13637	.	stem_loop	2240	2283	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_I13637
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatg
tccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaaccc
gtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgc
cttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgt
aataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacag
aaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgc
actcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaa
acacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcct
tcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacga
aaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgataga
gatactgagcactactagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgca
gagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaa
aagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgct
gattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaa
ctgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcg
cgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaa
tgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcga
ctgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgc
gtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggag
tgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgat
cagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacg
acgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccag
cgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaaga
aaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg
tttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtg
tagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattg
tgagcggataacaattgacattgtgagcggataacaagatactgagcaca