##gff-version 3
##sequence-region BBa_I13634 1 2422
BBa_I13634	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I13634	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13634	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13634	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13634	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13634	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13634	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13634	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13634	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13634	.	engineered_region	1050	2422	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13634	.	promoter	2368	2421	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13634	.	promoter	2410	2415	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13634	.	operator	2393	2409	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13634	.	operator	2370	2386	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13634	.	engineered_region	2368	2421	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13634	.	promoter	2387	2392	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13634	.	ribosome_entry_site	1050	1063	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13634	.	conserved_region	1056	1059	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13634	.	CDS	1070	2197	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13634	.	engineered_tag	2198	2222	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13634	.	CDS	1070	2197	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13634	.	tag	2159	2197	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13634	.	engineered_region	1070	2197	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13634	.	terminator	2231	2359	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13634	.	terminator	2231	2310	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13634	.	stem_loop	2242	2285	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13634	.	engineered_region	2231	2310	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13634	.	terminator	2319	2359	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13634	.	engineered_region	2319	2359	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13634	.	stem_loop	2326	2345	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13634	.	stop_codon	2352	2352	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13634	.	polyA_site	2346	2359	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13634	.	engineered_region	138	1041	.	+	0	ID=BBa_Q01400;Name=BBa_Q01400
BBa_I13634	.	ribosome_entry_site	138	151	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01400
BBa_I13634	.	conserved_region	144	147	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13634	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01400
BBa_I13634	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13634	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13634	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01400
BBa_I13634	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13634	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13634	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13634	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13634	.	promoter	988	1041	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q01400
BBa_I13634	.	promoter	1007	1012	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13634	.	non_covalent_binding_site	1013	1031	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13634	.	engineered_region	988	1041	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13634	.	non_covalent_binding_site	988	1006	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13634	.	promoter	1030	1035	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13634	.	CDS	158	817	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q01400
BBa_I13634	.	engineered_tag	818	842	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13634	.	CDS	161	777	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13634	.	tag	778	811	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_I13634
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacacaggaaacctactaga
tgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaac
ccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgac
gccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttac
gtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctac
agaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatat
gcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggg
aaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagc
cttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgac
gaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgata
gagatactgagcactactagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcg
cagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggga
aaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttg
ctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatc
aactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttct
cgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcact
aatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgc
gactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggc
gcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactgg
agtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacg
atcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggata
cgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaacc
agcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaa
gaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgaca
ggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaat
tgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca