##gff-version 3
##sequence-region BBa_I13631 1 2419
BBa_I13631	.	engineered_region	1047	2419	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13631	.	CDS	1067	2194	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13631	.	engineered_region	1067	2194	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13631	.	engineered_tag	2195	2219	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13631	.	CDS	1067	2194	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13631	.	tag	2156	2194	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13631	.	ribosome_entry_site	1047	1060	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13631	.	conserved_region	1053	1056	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13631	.	promoter	2365	2418	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13631	.	engineered_region	2365	2418	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13631	.	operator	2367	2383	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13631	.	promoter	2407	2412	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13631	.	operator	2390	2406	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13631	.	promoter	2384	2389	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13631	.	terminator	2228	2356	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13631	.	terminator	2228	2307	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13631	.	stem_loop	2239	2282	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13631	.	engineered_region	2228	2307	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13631	.	terminator	2316	2356	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13631	.	engineered_region	2316	2356	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13631	.	stop_codon	2349	2349	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13631	.	stem_loop	2323	2342	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13631	.	polyA_site	2343	2356	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13631	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I13631	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13631	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13631	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13631	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13631	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13631	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13631	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13631	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13631	.	engineered_region	138	1038	.	+	0	ID=BBa_Q03400;Name=BBa_Q03400
BBa_I13631	.	terminator	936	976	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q03400
BBa_I13631	.	engineered_region	936	976	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13631	.	stop_codon	969	969	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13631	.	stem_loop	943	962	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13631	.	polyA_site	963	976	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13631	.	ribosome_entry_site	138	148	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_Q03400
BBa_I13631	.	engineered_region	138	148	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_I13631	.	ribosome_entry_site	138	148	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_I13631	.	ribosome_entry_site	144	147	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_I13631	.	promoter	985	1038	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q03400
BBa_I13631	.	promoter	1004	1009	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13631	.	non_covalent_binding_site	1010	1028	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13631	.	engineered_region	985	1038	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13631	.	non_covalent_binding_site	985	1003	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13631	.	promoter	1027	1032	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13631	.	CDS	155	814	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q03400
BBa_I13631	.	engineered_tag	815	839	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13631	.	tag	775	808	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13631	.	CDS	158	774	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13631	.	terminator	848	927	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q03400
BBa_I13631	.	stem_loop	859	902	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13631	.	engineered_region	848	927	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I13631
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacacaggactactagatgt
ccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccg
taaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcc
ttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgta
ataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacaga
aaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgca
ctcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaa
cacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcctt
cttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaa
aactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagag
atactgagcactactagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcag
agtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaa
agtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctg
attggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaac
tgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgc
gcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaat
gttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgac
tgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcg
tctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagt
gccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatc
agatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacga
cgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagc
gtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa
aaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgt
gagcggataacaattgacattgtgagcggataacaagatactgagcaca