##gff-version 3
##sequence-region BBa_I13627 1 2283
BBa_I13627	.	engineered_region	1380	2283	.	+	0	ID=BBa_Q01400;Name=BBa_Q01400
BBa_I13627	.	terminator	2181	2221	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01400
BBa_I13627	.	polyA_site	2208	2221	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13627	.	stop_codon	2214	2214	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13627	.	stem_loop	2188	2207	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13627	.	engineered_region	2181	2221	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13627	.	terminator	2093	2172	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01400
BBa_I13627	.	stem_loop	2104	2147	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13627	.	engineered_region	2093	2172	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13627	.	CDS	1400	2059	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q01400
BBa_I13627	.	tag	2020	2053	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13627	.	CDS	1403	2019	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13627	.	engineered_tag	2060	2084	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13627	.	promoter	2230	2283	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q01400
BBa_I13627	.	non_covalent_binding_site	2255	2273	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13627	.	engineered_region	2230	2283	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13627	.	promoter	2249	2254	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13627	.	promoter	2272	2277	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13627	.	non_covalent_binding_site	2230	2248	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13627	.	ribosome_entry_site	1380	1393	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01400
BBa_I13627	.	conserved_region	1386	1389	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13627	.	engineered_region	1	1371	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_I13627	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_I13627	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13627	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_I13627	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13627	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13627	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13627	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13627	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13627	.	terminator	1180	1308	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_I13627	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13627	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13627	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13627	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13627	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13627	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13627	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13627	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13627	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_I13627	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13627	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13627	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13627	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
>BBa_I13627
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcacatactagagtcacacaggaaacctactagatgtccagattagataaaagtaa
agtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagcta
ggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttag
ataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttag
atgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctc
gaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcatt
ttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtat
gccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctt
aataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac