##gff-version 3
##sequence-region BBa_I13625 1 2283
BBa_I13625	.	engineered_region	1	1373	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13625	.	CDS	21	1148	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13625	.	engineered_tag	1149	1173	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13625	.	tag	1110	1148	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13625	.	engineered_region	21	1148	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13625	.	CDS	21	1148	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13625	.	promoter	1319	1372	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13625	.	operator	1321	1337	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13625	.	promoter	1338	1343	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13625	.	operator	1344	1360	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13625	.	promoter	1361	1366	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13625	.	engineered_region	1319	1372	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13625	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13625	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13625	.	terminator	1182	1310	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13625	.	terminator	1270	1310	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13625	.	polyA_site	1297	1310	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13625	.	engineered_region	1270	1310	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13625	.	stem_loop	1277	1296	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13625	.	stop_codon	1303	1303	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13625	.	terminator	1182	1261	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13625	.	engineered_region	1182	1261	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13625	.	stem_loop	1193	1236	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13625	.	engineered_region	1382	2283	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I13625	.	promoter	2230	2283	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I13625	.	engineered_region	2230	2283	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13625	.	promoter	2272	2277	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13625	.	non_covalent_binding_site	2230	2248	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13625	.	promoter	2249	2254	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13625	.	non_covalent_binding_site	2255	2273	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13625	.	CDS	1400	2059	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I13625	.	engineered_tag	2060	2084	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13625	.	CDS	1403	2019	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13625	.	tag	2020	2053	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13625	.	terminator	2181	2221	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I13625	.	polyA_site	2208	2221	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13625	.	engineered_region	2181	2221	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13625	.	stem_loop	2188	2207	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13625	.	stop_codon	2214	2214	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13625	.	ribosome_entry_site	1382	1393	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I13625	.	conserved_region	1386	1389	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13625	.	terminator	2093	2172	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I13625	.	engineered_region	2093	2172	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13625	.	stem_loop	2104	2147	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_I13625
tcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctta
tcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcg
gagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctcca
gtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggt
gtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctg
atcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttg
atgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggt
cgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctgg
cataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaac
aaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaat
gcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctca
tgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaac
tctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaa
tacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcggg
caggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgaca
ttgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaa
agtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagcta
ggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttag
ataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttag
atgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctc
gaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcatt
ttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtat
gccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctt
aataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac