##gff-version 3
##sequence-region BBa_I13613 1 2282
BBa_I13613	.	engineered_region	1	904	.	+	0	ID=BBa_Q01400;Name=BBa_Q01400
BBa_I13613	.	terminator	802	842	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01400
BBa_I13613	.	polyA_site	829	842	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13613	.	stem_loop	809	828	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13613	.	engineered_region	802	842	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13613	.	stop_codon	835	835	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13613	.	terminator	714	793	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01400
BBa_I13613	.	engineered_region	714	793	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13613	.	stem_loop	725	768	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13613	.	promoter	851	904	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q01400
BBa_I13613	.	non_covalent_binding_site	851	869	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13613	.	promoter	870	875	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13613	.	engineered_region	851	904	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13613	.	promoter	893	898	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13613	.	non_covalent_binding_site	876	894	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13613	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01400
BBa_I13613	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13613	.	CDS	21	680	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q01400
BBa_I13613	.	tag	641	674	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13613	.	engineered_tag	681	705	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13613	.	CDS	24	640	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13613	.	engineered_region	913	2282	.	+	0	ID=BBa_Q03121;Name=BBa_Q03121
BBa_I13613	.	CDS	930	2057	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q03121
BBa_I13613	.	engineered_tag	2058	2082	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13613	.	CDS	930	2057	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13613	.	engineered_region	930	2057	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13613	.	tag	2019	2057	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13613	.	ribosome_entry_site	913	923	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_Q03121
BBa_I13613	.	ribosome_entry_site	913	923	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_I13613	.	engineered_region	913	923	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_I13613	.	ribosome_entry_site	919	922	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_I13613	.	terminator	2091	2219	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q03121
BBa_I13613	.	terminator	2179	2219	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13613	.	polyA_site	2206	2219	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13613	.	stem_loop	2186	2205	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13613	.	engineered_region	2179	2219	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13613	.	stop_codon	2212	2212	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13613	.	terminator	2091	2170	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13613	.	engineered_region	2091	2170	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13613	.	stem_loop	2102	2145	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13613	.	promoter	2228	2281	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q03121
BBa_I13613	.	operator	2253	2269	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13613	.	operator	2230	2246	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13613	.	engineered_region	2228	2281	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13613	.	promoter	2247	2252	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13613	.	promoter	2270	2275	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
>BBa_I13613
tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggt
cggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaa
aataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggg
aaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagt
acatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttt
tcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatc
aagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttga
tcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaa
agtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagaga
ttgacatccctatcagtgatagagatactgagcactactagagtcacacaggactactagatggtgaatgtgaaaccag
taacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgt
ttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactg
gcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga
ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagc
ggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgct
gtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttct
cccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggccc
attaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcg
gaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactg
cgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgga
tatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatttt
cgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgc
ccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt
aatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaa
taactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca