##gff-version 3
##sequence-region BBa_I13611 1 2282
BBa_I13611	.	engineered_region	910	2282	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13611	.	CDS	930	2057	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13611	.	engineered_region	930	2057	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13611	.	engineered_tag	2058	2082	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13611	.	CDS	930	2057	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13611	.	tag	2019	2057	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13611	.	terminator	2091	2219	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13611	.	terminator	2179	2219	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13611	.	polyA_site	2206	2219	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13611	.	stop_codon	2212	2212	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13611	.	engineered_region	2179	2219	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13611	.	stem_loop	2186	2205	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13611	.	terminator	2091	2170	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13611	.	stem_loop	2102	2145	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13611	.	engineered_region	2091	2170	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13611	.	promoter	2228	2281	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13611	.	promoter	2247	2252	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13611	.	promoter	2270	2275	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13611	.	engineered_region	2228	2281	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13611	.	operator	2230	2246	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13611	.	operator	2253	2269	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13611	.	ribosome_entry_site	910	923	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13611	.	conserved_region	916	919	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13611	.	engineered_region	1	901	.	+	0	ID=BBa_Q03400;Name=BBa_Q03400
BBa_I13611	.	CDS	18	677	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q03400
BBa_I13611	.	CDS	21	637	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13611	.	tag	638	671	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13611	.	engineered_tag	678	702	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13611	.	terminator	711	790	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q03400
BBa_I13611	.	stem_loop	722	765	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13611	.	engineered_region	711	790	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13611	.	ribosome_entry_site	1	11	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_Q03400
BBa_I13611	.	engineered_region	1	11	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_I13611	.	ribosome_entry_site	1	11	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_I13611	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_I13611	.	promoter	848	901	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q03400
BBa_I13611	.	engineered_region	848	901	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13611	.	promoter	890	895	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13611	.	non_covalent_binding_site	873	891	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13611	.	promoter	867	872	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13611	.	non_covalent_binding_site	848	866	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13611	.	terminator	799	839	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q03400
BBa_I13611	.	polyA_site	826	839	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13611	.	stop_codon	832	832	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13611	.	engineered_region	799	839	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13611	.	stem_loop	806	825	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I13611
tcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcgg
aatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaat
aagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaa
gctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtaca
tttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttca
ctagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaag
tcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatca
ccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagt
gggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattg
acatccctatcagtgatagagatactgagcactactagagtcacacaggaaacctactagatggtgaatgtgaaaccag
taacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgt
ttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactg
gcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga
ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagc
ggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgct
gtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttct
cccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggccc
attaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcg
gaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactg
cgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgga
tatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatttt
cgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgc
ccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt
aatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaa
taactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca