##gff-version 3
##sequence-region BBa_I13581 1 2264
BBa_I13581	.	sequence_feature	1502	1507	.	+	0	ID=BBa_M31991;Name=BBa_M31991
BBa_I13581	.	sequence_feature	1078	1083	.	+	0	ID=BBa_M31992;Name=BBa_M31992
BBa_I13581	.	sequence_feature	2251	2256	.	+	0	ID=BBa_B0046;Name=BBa_B0046
BBa_I13581	.	sequence_feature	2251	2256	.	+	0	ID=annotation1822730;Name=NsiI site;Parent=BBa_B0046
BBa_I13581	.	sequence_feature	2257	2264	.	+	0	ID=BBa_G0008;Name=BBa_G0008
BBa_I13581	.	sequence_feature	1	8	.	+	0	ID=BBa_G0007;Name=BBa_G0007
BBa_I13581	.	sequence_feature	9	14	.	+	0	ID=BBa_B0047;Name=BBa_B0047
BBa_I13581	.	sequence_feature	9	14	.	+	0	ID=annotation1822740;Name=MfeI site;Parent=BBa_B0047
BBa_I13581	.	sequence_feature	1524	1529	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_I13581	.	engineered_region	1085	1500	.	+	0	ID=BBa_P1016;Name=BBa_P1016
BBa_I13581	.	promoter	1125	1130	.	+	0	ID=annotation1910684;Name=-35;Parent=BBa_P1016
BBa_I13581	.	promoter	1148	1153	.	+	0	ID=annotation1910685;Name=-10;Parent=BBa_P1016
BBa_I13581	.	CDS	1192	1500	.	+	0	ID=annotation1910686;Name=ccdB;Parent=BBa_P1016
BBa_I13581	.	CDS	1530	2249	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I13581	.	CDS	1530	2249	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I13581	.	ribosome_entry_site	1509	1523	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I13581	.	engineered_region	1509	1523	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I13581	.	ribosome_entry_site	1516	1520	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I13581	.	ribosome_entry_site	1509	1523	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I13581	.	engineered_region	16	1076	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_I13581	.	ribosome_entry_site	78	89	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_I13581	.	conserved_region	82	85	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13581	.	promoter	16	69	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_I13581	.	promoter	35	40	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13581	.	non_covalent_binding_site	16	34	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13581	.	engineered_region	16	69	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13581	.	promoter	58	63	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13581	.	non_covalent_binding_site	41	59	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13581	.	terminator	885	1013	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_I13581	.	terminator	973	1013	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13581	.	stem_loop	980	999	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13581	.	engineered_region	973	1013	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13581	.	stop_codon	1006	1006	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13581	.	polyA_site	1000	1013	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13581	.	terminator	885	964	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13581	.	engineered_region	885	964	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13581	.	stem_loop	896	939	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13581	.	CDS	96	851	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_I13581	.	engineered_tag	852	876	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13581	.	engineered_region	96	97	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13581	.	CDS	96	845	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13581	.	point_mutation	269	269	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13581	.	engineered_region	96	851	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13581	.	point_mutation	587	587	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13581	.	promoter	1022	1076	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_I13581	.	non_covalent_binding_site	1022	1041	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13581	.	promoter	1041	1046	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13581	.	engineered_region	1022	1076	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13581	.	promoter	1063	1068	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13581	.	start_codon	1074	1074	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
>BBa_I13581
gtttcttccaattggtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaa
agaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagc
aataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatc
ctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaa
tttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaat
gctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattc
atacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttaca
tgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatca
aacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaa
tattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcca
aagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgt
aggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagtaggcttactaaaagccagataac
agtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagagg
tatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatatta
ttgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaacttta
cccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggg
gaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataac
tctagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatatgcatgaagaaac