##gff-version 3
##sequence-region BBa_I13552 1 2146
BBa_I13552	.	engineered_region	1	1158	.	+	0	ID=BBa_F2621;Name=BBa_F2621
BBa_I13552	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_I13552	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13552	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13552	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13552	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13552	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_I13552	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I13552	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I13552	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I13552	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I13552	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I13552	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I13552	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_I13552	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13552	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13552	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_I13552	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13552	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13552	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13552	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13552	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13552	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_I13552	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13552	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13552	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13552	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13552	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13552	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13552	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_I13552	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13552	.	engineered_region	1167	2146	.	+	0	ID=BBa_I13528;Name=BBa_I13528
BBa_I13552	.	engineered_region	1286	2146	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_I13528
BBa_I13552	.	terminator	2106	2146	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_I13552	.	stop_codon	2139	2139	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13552	.	polyA_site	2133	2146	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13552	.	stem_loop	2113	2132	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13552	.	engineered_region	2106	2146	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13552	.	ribosome_entry_site	1286	1297	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_I13552	.	conserved_region	1290	1293	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13552	.	terminator	2018	2097	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_I13552	.	stem_loop	2029	2072	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13552	.	engineered_region	2018	2097	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13552	.	CDS	1304	1984	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_I13552	.	CDS	1304	1978	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13552	.	engineered_tag	1985	2009	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13552	.	sequence_feature	1167	1277	.	+	0	ID=BBa_I13457;Name=BBa_I13457;Parent=BBa_I13528
BBa_I13552	.	sequence_feature	1175	1176	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13552	.	sequence_feature	1188	1221	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13552	.	stem_loop	1243	1277	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13552	.	sequence_feature	1167	1187	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13552	.	sequence_feature	1230	1231	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13552	.	sequence_feature	1222	1242	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
>BBa_I13552
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtattatttgtattgatctg
gtgaattaggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagt
acctttggagatctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtt
tcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaagg
tacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccag
tacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaat
gggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcat
ctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagct
tccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtc
actacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaa
actggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata