##gff-version 3
##sequence-region BBa_I13544 1 2240
BBa_I13544	.	ribosome_entry_site	1380	1391	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13544	.	conserved_region	1384	1387	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13544	.	promoter	1123	1252	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_I13544	.	sequence_feature	1261	1371	.	+	0	ID=BBa_I13457;Name=BBa_I13457
BBa_I13544	.	sequence_feature	1316	1336	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13544	.	stem_loop	1337	1371	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13544	.	sequence_feature	1324	1325	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13544	.	sequence_feature	1269	1270	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13544	.	sequence_feature	1282	1315	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13544	.	sequence_feature	1261	1281	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13544	.	CDS	1398	2078	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_I13544	.	engineered_tag	2079	2103	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13544	.	CDS	1398	2072	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13544	.	terminator	2112	2191	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13544	.	engineered_region	2112	2191	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13544	.	stem_loop	2123	2166	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13544	.	engineered_region	1	1114	.	+	0	ID=BBa_I13458;Name=BBa_I13458
BBa_I13544	.	terminator	2200	2240	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13544	.	polyA_site	2227	2240	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13544	.	engineered_region	2200	2240	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13544	.	stop_codon	2233	2233	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13544	.	stem_loop	2207	2226	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I13544
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattag
cggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtatt
atttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttactatc
tctcgagagattagtacctttggagatctactagagaaagaggagaaatactagatggcttcctccgaagacgttatca
aagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaagg
tcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctg
tccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcc
cggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgca
agacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacc
atgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaac
tgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctta
caaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggt
cgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata