##gff-version 3
##sequence-region BBa_I13543 1 2198
BBa_I13543	.	sequence_feature	1219	1329	.	+	0	ID=BBa_I13457;Name=BBa_I13457
BBa_I13543	.	sequence_feature	1219	1239	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13543	.	sequence_feature	1240	1273	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13543	.	stem_loop	1295	1329	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13543	.	sequence_feature	1274	1294	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13543	.	sequence_feature	1227	1228	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13543	.	sequence_feature	1282	1283	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13543	.	terminator	2070	2149	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13543	.	stem_loop	2081	2124	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13543	.	engineered_region	2070	2149	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13543	.	ribosome_entry_site	1338	1349	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13543	.	conserved_region	1342	1345	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13543	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I13543	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I13543	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I13543	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I13543	.	CDS	1356	2036	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_I13543	.	CDS	1356	2030	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13543	.	engineered_tag	2037	2061	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13543	.	terminator	2158	2198	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13543	.	engineered_region	2158	2198	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13543	.	stem_loop	2165	2184	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13543	.	stop_codon	2191	2191	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13543	.	polyA_site	2185	2198	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_I13543
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagtattatttgtattgatctggtgaattaggtcttcgaatccgatcca
ttgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatctactagagaaagag
gagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgtta
acggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttac
caaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacac
ccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacg
gtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaa
cttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
ggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacct
acatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaaga
ctacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgt
agatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata