##gff-version 3
##sequence-region BBa_I13527 1 994
BBa_I13527	.	engineered_region	120	994	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_I13527	.	ribosome_entry_site	120	131	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_I13527	.	conserved_region	124	127	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13527	.	terminator	866	945	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_I13527	.	engineered_region	866	945	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13527	.	stem_loop	877	920	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13527	.	terminator	954	994	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_I13527	.	stem_loop	961	980	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13527	.	engineered_region	954	994	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13527	.	stop_codon	987	987	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13527	.	polyA_site	981	994	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13527	.	CDS	138	857	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_I13527	.	CDS	138	857	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I13527	.	sequence_feature	1	111	.	+	0	ID=BBa_I13457;Name=BBa_I13457
BBa_I13527	.	sequence_feature	64	65	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13527	.	sequence_feature	9	10	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13527	.	sequence_feature	1	21	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13527	.	stem_loop	77	111	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13527	.	sequence_feature	56	76	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13527	.	sequence_feature	22	55	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
>BBa_I13527
tattatttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttac
tatctctcgagagattagtacctttggagatctactagagaaagaggagaaatactagatgcgtaaaggagaagaactt
ttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg
aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacact
tgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagt
gccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtca
agtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggaca
caaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttc
aaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggcc
ctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacat
ggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata