##gff-version 3
##sequence-region BBa_I13213 1 1964
BBa_I13213	.	terminator	1836	1915	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13213	.	stem_loop	1847	1890	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13213	.	engineered_region	1836	1915	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13213	.	CDS	1047	1802	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I13213	.	engineered_tag	1803	1827	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13213	.	engineered_region	1047	1802	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13213	.	engineered_region	1047	1048	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13213	.	point_mutation	1220	1220	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13213	.	point_mutation	1538	1538	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13213	.	CDS	1047	1796	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13213	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I13213	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13213	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13213	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13213	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13213	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13213	.	terminator	1924	1964	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13213	.	polyA_site	1951	1964	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13213	.	engineered_region	1924	1964	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13213	.	stop_codon	1957	1957	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13213	.	stem_loop	1931	1950	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13213	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13213	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13213	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13213	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13213	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13213	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13213	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13213	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13213	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13213	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13213	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I13213	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I13213	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I13213	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I13213	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I13213	.	ribosome_entry_site	1029	1040	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13213	.	conserved_region	1033	1036	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13213	.	promoter	870	1020	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I13213	.	engineered_region	870	1020	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I13213	.	non_covalent_binding_site	870	889	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I13213	.	promoter	984	991	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I13213	.	start_codon	997	997	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I13213	.	non_covalent_binding_site	999	1018	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I13213	.	promoter	958	963	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
>BBa_I13213
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata