LOCUS       BBa_I13213              1964 bp    DNA     linear       15-Jul-2025
DEFINITION  BioBricked version of the natural Lux system with order reversed
ACCESSION   BBa_I13213
VERSION     BBa_I13213.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     stem_loop       744..787
                     /label=stem_loop
     misc_feature    733..812
                     /label=BBa_B0010
     terminator      733..812
                     /label=BBa_B0010
     stem_loop       828..847
                     /label=T7 TE
     misc_feature    854..854
                     /label=stop
     polyA_site      848..861
                     /label=polya
     misc_feature    821..861
                     /label=BBa_B0012
     terminator      821..861
                     /label=BBa_B0012
     misc_feature    870..889
                     /label=LuxR/HSL
     promoter        984..991
                     /label=-10
     promoter        958..963
                     /label=-35
     misc_feature    997..997
                     /label=start
     misc_feature    999..1018
                     /label=Putative LuxR/HSL
     misc_feature    870..1020
                     /label=BBa_R0063
     promoter        870..1020
                     /label=BBa_R0063
     misc_feature    1033..1036
                     /label=conserved
     RBS             1029..1040
                     /label=BBa_B0034
     misc_feature    1047..1048
                     /label=prefix
     misc_feature    1220..1220
                     /label=T
     misc_feature    1538..1538
                     /label=A
     CDS             1047..1796
                     /label=luxR
     misc_feature    1047..1802
                     /label=BBa_C0062
     misc_feature    1803..1827
                     /label=Help:Barcodes
     CDS             1047..1802
                     /label=BBa_C0062
     stem_loop       1847..1890
                     /label=stem_loop
     misc_feature    1836..1915
                     /label=BBa_B0010
     terminator      1836..1915
                     /label=BBa_B0010
     stem_loop       1931..1950
                     /label=T7 TE
     misc_feature    1957..1957
                     /label=stop
     polyA_site      1951..1964
                     /label=polya
     misc_feature    1924..1964
                     /label=BBa_B0012
     terminator      1924..1964
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      841 gtgggccttt ctgcgtttat atactagaga cctgtacgat cctacaggtg cttatgttaa
      901 gtaattgtat tcccagcgat acaatagtgt gacaaaaatc caatttatta gaatcaaatg
      961 tcaatccatt accgttttaa tgatatataa cacgcaaaac ttgcgacaaa caataggtaa
     1021 tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac acatacagaa
     1081 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata
     1141 tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg
     1201 ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa tattatgatg
     1261 acgctaattt aataaaatat gatcctatag tagattattc taactccaat cattcaccaa
     1321 ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag
     1381 aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg
     1441 gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt
     1501 tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa
     1561 taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag
     1621 cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt tgcagtgagc
     1681 gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc
     1741 aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaat
     1801 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc
     1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//