##gff-version 3
##sequence-region BBa_I13115 1 1925
BBa_I13115	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13115	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13115	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13115	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13115	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13115	.	terminator	937	977	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13115	.	stem_loop	944	963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13115	.	stop_codon	970	970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13115	.	polyA_site	964	977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13115	.	engineered_region	937	977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13115	.	terminator	1885	1925	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13115	.	stem_loop	1892	1911	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13115	.	stop_codon	1918	1918	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13115	.	polyA_site	1912	1925	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13115	.	engineered_region	1885	1925	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13115	.	CDS	156	815	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I13115	.	tag	776	809	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13115	.	engineered_tag	816	840	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13115	.	CDS	159	775	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13115	.	promoter	986	1039	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13115	.	engineered_region	986	1039	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13115	.	promoter	1005	1010	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13115	.	promoter	1028	1033	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13115	.	non_covalent_binding_site	986	1004	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13115	.	non_covalent_binding_site	1011	1029	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13115	.	ribosome_entry_site	1048	1059	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13115	.	conserved_region	1052	1055	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13115	.	CDS	1066	1788	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I13115	.	terminator	1797	1876	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13115	.	stem_loop	1808	1851	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13115	.	engineered_region	1797	1876	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13115	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13115	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13115	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13115	.	terminator	849	928	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13115	.	stem_loop	860	903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13115	.	engineered_region	849	928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13115	.	ribosome_entry_site	138	149	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13115	.	conserved_region	142	145	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13115
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatg
tccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaaccc
gtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgc
cttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgt
aataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacag
aaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgc
actcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaa
acacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcct
tcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacga
aaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgataga
gatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcc
catcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctac
ggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggct
acggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggcta
cgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacacc
ctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaact
acaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacat
cgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgac
aaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcg
tgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata