##gff-version 3
##sequence-region BBa_I13105 1 1853
BBa_I13105	.	engineered_region	64	1853	.	+	0	ID=BBa_I13023;Name=BBa_I13023
BBa_I13105	.	CDS	994	1716	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I13023
BBa_I13105	.	ribosome_entry_site	64	77	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_I13023
BBa_I13105	.	conserved_region	70	73	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13105	.	terminator	1813	1853	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13023
BBa_I13105	.	polyA_site	1840	1853	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13105	.	engineered_region	1813	1853	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13105	.	stem_loop	1820	1839	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13105	.	stop_codon	1846	1846	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13105	.	CDS	84	743	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I13023
BBa_I13105	.	engineered_tag	744	768	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13105	.	CDS	87	703	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13105	.	tag	704	737	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13105	.	promoter	914	967	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13023
BBa_I13105	.	engineered_region	914	967	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13105	.	non_covalent_binding_site	914	932	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13105	.	promoter	956	961	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13105	.	promoter	933	938	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13105	.	non_covalent_binding_site	939	957	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13105	.	terminator	865	905	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13023
BBa_I13105	.	polyA_site	892	905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13105	.	engineered_region	865	905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13105	.	stem_loop	872	891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13105	.	stop_codon	898	898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13105	.	ribosome_entry_site	976	987	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13023
BBa_I13105	.	conserved_region	980	983	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13105	.	terminator	1725	1804	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13023
BBa_I13105	.	engineered_region	1725	1804	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13105	.	stem_loop	1736	1779	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13105	.	terminator	777	856	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13023
BBa_I13105	.	engineered_region	777	856	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13105	.	stem_loop	788	831	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13105	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I13105	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13105	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13105	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13105	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13105	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_I13105
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaaccta
ctagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttta
acaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgc
tcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattt
tttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacgg
cctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcat
tatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaaga
aagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagag
ccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaa
acgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccgggg
tggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgc
cacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacc
ttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccg
aaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgaggg
cgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggag
tacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgcc
acaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgct
gcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctg
gagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata