LOCUS       BBa_I13104              1878 bp    DNA     linear       21-Aug-2025
DEFINITION  Low output control (c2 QPI)
ACCESSION   BBa_I13104
VERSION     BBa_I13104.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..729
                     /label=cII p22
     misc_feature    730..762
                     /label=LVA
     misc_feature    763..768
                     /label=stop
     misc_feature    82..768
                     /label=BBa_C0053
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             82..768
                     /label=BBa_C0053
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     misc_feature    939..941
                     /label=OR3
     misc_feature    949..966
                     /label=OR2
     misc_feature    972..989
                     /label=OR1
     promoter        956..961
                     /label=-35
     promoter        946..951
                     /label=-35
     promoter        968..973
                     /label=-10
     misc_feature    939..992
                     /label=BBa_R0053
     promoter        939..992
                     /label=BBa_R0053
     misc_feature    1005..1008
                     /label=conserved
     RBS             1001..1012
                     /label=BBa_B0034
     CDS             1019..1741
                     /label=BBa_E0030
     stem_loop       1761..1804
                     /label=stem_loop
     misc_feature    1750..1829
                     /label=BBa_B0010
     terminator      1750..1829
                     /label=BBa_B0010
     stem_loop       1845..1864
                     /label=T7 TE
     misc_feature    1871..1871
                     /label=stop
     polyA_site      1865..1878
                     /label=polya
     misc_feature    1838..1878
                     /label=BBa_B0012
     terminator      1838..1878
                     /label=BBa_B0012
     misc_feature    64..1878
                     /label=BBa_I13020
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaataca caattgatgg gtgagcgtat tcgcgctcga
      121 agaaaaaaac tcaagattag acaagccgct cttggtaaga tggtgggagt gtctaatgtt
      181 gcaatatcgc aatgggagcg ctcggagact gagccaaatg gggagaacct gttggcactt
      241 tcgaaggctc ttcagtgctc ccctgactat ttgctgaaag gagatttaag ccagacaaac
      301 gttgcctatc atagtaggca tgagccaaga ggatcatacc ctcttatcag ttgggtaagc
      361 gcagggcaat ggatggaagc tgtagaacct tatcacaagc gcgcgataga gaactggcac
      421 gacaccactg tagattgttc agaagattca ttttggcttg atgtccaagg tgactctatg
      481 acagcaccgg cagggttaag cattccagaa ggaatgataa ttctggttga tcccgaagtc
      541 gaaccaagaa acggcaagct ggttgttgca aaattagaag gtgaaaacga ggccacattc
      601 aaaaaattag ttatggatgc aggccgaaag tttttaaaac cattaaaccc acaatatccg
      661 atgatagaaa tcaacggaaa ctgcaaaatc attggcgtag ttgttgacgc aaaactcgca
      721 aatcttccag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
      781 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagaa taaacttgac taaagattcc
      961 tttagtagat aatttaagtg ttctttaatt tctactagag aaagaggaga aatactagat
     1021 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1081 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1141 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1201 cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct
     1261 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1321 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1381 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1441 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1501 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1561 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1621 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1681 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata
     1741 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1801 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1861 ggcctttctg cgtttata
//