LOCUS       BBa_I13102              2320 bp    DNA     linear       23-Jun-2025
DEFINITION  Lac Self-coupled Inverter
ACCESSION   BBa_I13102
VERSION     BBa_I13102.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1171..1209
                     /label=LVA
     CDS             82..1209
                     /label=lacI-LVA
     misc_feature    82..1209
                     /label=BBa_C0012
     misc_feature    1210..1234
                     /label=Help:Barcodes
     CDS             82..1209
                     /label=BBa_C0012
     stem_loop       1254..1297
                     /label=stem_loop
     misc_feature    1243..1322
                     /label=BBa_B0010
     terminator      1243..1322
                     /label=BBa_B0010
     stem_loop       1338..1357
                     /label=T7 TE
     misc_feature    1364..1364
                     /label=stop
     polyA_site      1358..1371
                     /label=polya
     misc_feature    1331..1371
                     /label=BBa_B0012
     terminator      1331..1371
                     /label=BBa_B0012
     terminator      1243..1371
                     /label=BBa_B0015
     misc_feature    1382..1398
                     /label=lac O1
     promoter        1399..1404
                     /label=-35
     misc_feature    1405..1421
                     /label=lac O1
     promoter        1422..1427
                     /label=-10
     misc_feature    1380..1433
                     /label=BBa_R0011
     promoter        1380..1433
                     /label=BBa_R0011
     misc_feature    64..1434
                     /label=BBa_Q04121
     misc_feature    1447..1450
                     /label=conserved
     RBS             1443..1454
                     /label=BBa_B0034
     CDS             1461..2183
                     /label=BBa_E0030
     stem_loop       2203..2246
                     /label=stem_loop
     misc_feature    2192..2271
                     /label=BBa_B0010
     terminator      2192..2271
                     /label=BBa_B0010
     stem_loop       2287..2306
                     /label=T7 TE
     misc_feature    2313..2313
                     /label=stop
     polyA_site      2307..2320
                     /label=polya
     misc_feature    2280..2320
                     /label=BBa_B0012
     terminator      2280..2320
                     /label=BBa_B0012
     misc_feature    1443..2320
                     /label=BBa_E0430
     misc_feature    64..2320
                     /label=BBa_I13021
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca
      121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
      661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
      721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
      781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
      841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
      901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
      961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta
     1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac
     1261 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1321 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
     1381 attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag cacatactag
     1441 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1501 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1561 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1621 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc
     1681 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg
     1741 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1801 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1861 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1921 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1981 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2041 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2101 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2161 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     2221 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2281 cacactggct caccttcggg tgggcctttc tgcgtttata
//