##gff-version 3
##sequence-region BBa_I13101 1 1877
BBa_I13101	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I13101	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I13101	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I13101	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I13101	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I13101	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I13101	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I13101	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I13101	.	engineered_region	63	1877	.	+	0	ID=BBa_I13020;Name=BBa_I13020
BBa_I13101	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13020
BBa_I13101	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13101	.	terminator	1749	1828	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13020
BBa_I13101	.	engineered_region	1749	1828	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13101	.	stem_loop	1760	1803	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13101	.	ribosome_entry_site	1000	1011	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13020
BBa_I13101	.	conserved_region	1004	1007	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13101	.	terminator	1837	1877	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13020
BBa_I13101	.	polyA_site	1864	1877	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13101	.	stem_loop	1844	1863	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13101	.	engineered_region	1837	1877	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13101	.	stop_codon	1870	1870	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13101	.	CDS	81	767	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_I13020
BBa_I13101	.	engineered_tag	768	792	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_I13101	.	stop_codon	762	767	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_I13101	.	tag	729	761	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_I13101	.	CDS	81	728	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_I13101	.	engineered_region	81	767	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_I13101	.	terminator	801	880	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13020
BBa_I13101	.	engineered_region	801	880	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13101	.	stem_loop	812	855	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13101	.	CDS	1018	1740	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I13020
BBa_I13101	.	promoter	938	991	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_I13020
BBa_I13101	.	promoter	955	960	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I13101	.	promoter	945	950	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I13101	.	non_covalent_binding_site	938	940	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I13101	.	engineered_region	938	991	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I13101	.	promoter	967	972	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I13101	.	non_covalent_binding_site	948	965	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I13101	.	non_covalent_binding_site	971	988	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I13101	.	terminator	889	929	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13020
BBa_I13101	.	polyA_site	916	929	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13101	.	stem_loop	896	915	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13101	.	engineered_region	889	929	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13101	.	stop_codon	922	922	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_I13101
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatacta
gatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaag
atggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcac
tttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtag
gcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcac
aagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgact
ctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacgg
caagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagttt
ttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaa
aactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatc
actactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttga
ctaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatactagatggtgagca
agggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgt
gtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtg
ccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacg
acttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagac
ccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggc
aacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggca
tcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccc
catcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgag
aagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata