##gff-version 3
##sequence-region BBa_I13038 1 6037
BBa_I13038	.	terminator	5997	6037	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13038	.	polyA_site	6024	6037	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13038	.	stop_codon	6030	6030	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13038	.	stem_loop	6004	6023	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13038	.	engineered_region	5997	6037	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13038	.	terminator	1888	1967	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13038	.	stem_loop	1899	1942	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13038	.	engineered_region	1888	1967	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13038	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13038	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13038	.	promoter	2025	2078	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13038	.	non_covalent_binding_site	2050	2068	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13038	.	engineered_region	2025	2078	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13038	.	promoter	2067	2072	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13038	.	non_covalent_binding_site	2025	2043	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13038	.	promoter	2044	2049	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13038	.	terminator	2894	2973	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13038	.	stem_loop	2905	2948	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13038	.	engineered_region	2894	2973	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13038	.	terminator	1976	2016	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13038	.	polyA_site	2003	2016	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13038	.	stop_codon	2009	2009	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13038	.	stem_loop	1983	2002	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13038	.	engineered_region	1976	2016	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13038	.	ribosome_entry_site	3094	3105	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13038	.	conserved_region	3098	3101	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13038	.	CDS	3112	3834	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I13038	.	CDS	1237	1854	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I13038	.	engineered_region	1237	1854	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I13038	.	CDS	1237	1815	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I13038	.	engineered_tag	1855	1879	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I13038	.	tag	1816	1847	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I13038	.	ribosome_entry_site	5198	5209	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13038	.	conserved_region	5202	5205	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13038	.	terminator	3931	3971	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13038	.	polyA_site	3958	3971	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13038	.	stop_codon	3964	3964	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13038	.	stem_loop	3938	3957	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13038	.	engineered_region	3931	3971	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13038	.	CDS	2105	2860	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I13038	.	engineered_tag	2861	2885	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13038	.	point_mutation	2278	2278	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13038	.	engineered_region	2105	2860	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13038	.	engineered_region	2105	2106	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13038	.	point_mutation	2596	2596	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13038	.	CDS	2105	2854	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13038	.	terminator	3843	3922	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13038	.	stem_loop	3854	3897	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13038	.	engineered_region	3843	3922	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13038	.	promoter	3031	3085	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I13038	.	engineered_region	3031	3085	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13038	.	start_codon	3083	3083	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13038	.	promoter	3072	3077	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13038	.	promoter	3050	3055	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13038	.	non_covalent_binding_site	3031	3050	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13038	.	promoter	3980	5189	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I13038	.	promoter	5059	5189	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I13038	.	promoter	5022	5093	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I13038	.	sequence_feature	3980	5058	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I13038	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I13038	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I13038	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I13038	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I13038	.	terminator	5909	5988	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13038	.	stem_loop	5920	5963	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13038	.	engineered_region	5909	5988	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13038	.	ribosome_entry_site	2087	2098	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13038	.	conserved_region	2091	2094	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13038	.	terminator	2982	3022	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13038	.	polyA_site	3009	3022	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13038	.	stop_codon	3015	3015	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13038	.	stem_loop	2989	3008	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13038	.	engineered_region	2982	3022	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13038	.	CDS	5216	5875	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I13038	.	CDS	5219	5835	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13038	.	tag	5836	5869	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13038	.	engineered_tag	5876	5900	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_I13038
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggatt
ttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtggga
cttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatact
gaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttg
gtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaa
taactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatat
gtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaa
ttcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgc
tgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccct
atcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacata
cagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggta
cattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattacc
ctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatca
ttcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaaca
tcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcag
aaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataa
ttatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggca
tgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatg
cgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccata
ctttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaa
taaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctgg
tcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagct
gaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctg
caatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccagg
agcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaa
ccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagc
cacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacg
gcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccacta
cctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgcc
gccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcac
ggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcg
accgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagctt
aagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctgg
cgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatg
gagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgc
ccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaaga
accccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtg
ataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcgg
caaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattccca
gcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacga
gtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgt
ccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttatta
aaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagt
ccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctga
cgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata