##gff-version 3
##sequence-region BBa_I13035 1 3003
BBa_I13035	.	terminator	1819	1898	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13035	.	engineered_region	1819	1898	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13035	.	stem_loop	1830	1873	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13035	.	ribosome_entry_site	1070	1081	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13035	.	conserved_region	1074	1077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13035	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13035	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13035	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13035	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13035	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13035	.	CDS	2182	2841	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I13035	.	engineered_tag	2842	2866	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13035	.	CDS	2185	2801	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13035	.	tag	2802	2835	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13035	.	terminator	2875	2954	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13035	.	engineered_region	2875	2954	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13035	.	stem_loop	2886	2929	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13035	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I13035	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13035	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13035	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13035	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13035	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13035	.	CDS	1088	1810	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I13035	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13035	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13035	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13035	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13035	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13035	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13035	.	terminator	2963	3003	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13035	.	polyA_site	2990	3003	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13035	.	engineered_region	2963	3003	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13035	.	stop_codon	2996	2996	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13035	.	stem_loop	2970	2989	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13035	.	terminator	1907	1947	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13035	.	polyA_site	1934	1947	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13035	.	engineered_region	1907	1947	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13035	.	stop_codon	1940	1940	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13035	.	stem_loop	1914	1933	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13035	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13035	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13035	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13035	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13035	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13035	.	promoter	1956	2155	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I13035	.	non_covalent_binding_site	2044	2081	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I13035	.	promoter	2116	2121	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I13035	.	start_codon	2128	2128	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I13035	.	engineered_region	1956	2155	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I13035	.	non_covalent_binding_site	2121	2155	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I13035	.	CDS	1956	2043	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I13035	.	promoter	2092	2097	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I13035	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I13035	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13035	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13035	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13035	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13035	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13035	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13035	.	ribosome_entry_site	2164	2175	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13035	.	conserved_region	2168	2171	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13035
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgagg
agctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcga
gggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc
accctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttca
agtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccga
ggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtga
acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcga
cggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgat
cacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctc
cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa
ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgt
gagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgatta
acagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtaga
gcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcac
catactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctt
tactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatca
attagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttta
ggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccat
tattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatg
cggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacac
tgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a