##gff-version 3
##sequence-region BBa_I13024 1 2259
BBa_I13024	.	engineered_region	1382	2259	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_I13024	.	terminator	2131	2210	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I13024	.	stem_loop	2142	2185	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13024	.	engineered_region	2131	2210	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13024	.	CDS	1400	2122	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I13024	.	terminator	2219	2259	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I13024	.	stop_codon	2252	2252	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13024	.	stem_loop	2226	2245	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13024	.	polyA_site	2246	2259	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13024	.	engineered_region	2219	2259	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13024	.	ribosome_entry_site	1382	1393	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I13024	.	conserved_region	1386	1389	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13024	.	engineered_region	1	1373	.	+	0	ID=BBa_Q01121;Name=BBa_Q01121
BBa_I13024	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01121
BBa_I13024	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13024	.	promoter	1319	1372	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q01121
BBa_I13024	.	operator	1321	1337	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13024	.	promoter	1361	1366	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13024	.	promoter	1338	1343	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13024	.	operator	1344	1360	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13024	.	engineered_region	1319	1372	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13024	.	terminator	1182	1310	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q01121
BBa_I13024	.	terminator	1182	1261	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13024	.	stem_loop	1193	1236	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13024	.	engineered_region	1182	1261	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13024	.	terminator	1270	1310	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13024	.	stop_codon	1303	1303	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13024	.	stem_loop	1277	1296	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13024	.	polyA_site	1297	1310	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13024	.	engineered_region	1270	1310	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13024	.	CDS	21	1148	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q01121
BBa_I13024	.	engineered_tag	1149	1173	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13024	.	engineered_region	21	1148	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13024	.	tag	1110	1148	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13024	.	CDS	21	1148	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
>BBa_I13024
tcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctta
tcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcg
gagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctcca
gtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggt
gtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctg
atcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttg
atgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggt
cgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctgg
cataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaac
aaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaat
gcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctca
tgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaac
tctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaa
tacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcggg
caggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgaca
ttgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggagct
gttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggc
gagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccc
tcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtc
cgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtg
aagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggc
acaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaactt
caagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggc
cccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcaca
tggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata