##gff-version 3
##sequence-region BBa_I13023 1 1790
BBa_I13023	.	terminator	714	793	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13023	.	engineered_region	714	793	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13023	.	stem_loop	725	768	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13023	.	terminator	1662	1741	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I13023	.	engineered_region	1662	1741	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13023	.	stem_loop	1673	1716	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13023	.	CDS	21	680	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I13023	.	engineered_tag	681	705	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I13023	.	CDS	24	640	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I13023	.	tag	641	674	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I13023	.	promoter	851	904	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I13023	.	non_covalent_binding_site	851	869	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13023	.	non_covalent_binding_site	876	894	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13023	.	promoter	870	875	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13023	.	engineered_region	851	904	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13023	.	promoter	893	898	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13023	.	terminator	802	842	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13023	.	polyA_site	829	842	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13023	.	stem_loop	809	828	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13023	.	engineered_region	802	842	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13023	.	stop_codon	835	835	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13023	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_I13023	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_I13023	.	terminator	1750	1790	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I13023	.	polyA_site	1777	1790	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13023	.	stem_loop	1757	1776	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13023	.	engineered_region	1750	1790	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13023	.	stop_codon	1783	1783	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13023	.	CDS	931	1653	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I13023	.	ribosome_entry_site	913	924	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I13023	.	conserved_region	917	920	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13023
tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggt
cggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaa
aataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggg
aaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagt
acatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttt
tcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatc
aagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttga
tcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaa
agtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagaga
ttgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgag
gagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcg
agggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcc
caccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttc
aagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccg
aggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcct
ggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtg
aacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcg
acggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcga
tcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata