##gff-version 3
##sequence-region BBa_I13021 1 2257
BBa_I13021	.	engineered_region	1380	2257	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_I13021	.	CDS	1398	2120	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I13021	.	terminator	2217	2257	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I13021	.	stem_loop	2224	2243	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13021	.	engineered_region	2217	2257	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13021	.	stop_codon	2250	2250	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13021	.	polyA_site	2244	2257	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13021	.	ribosome_entry_site	1380	1391	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I13021	.	conserved_region	1384	1387	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13021	.	terminator	2129	2208	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I13021	.	engineered_region	2129	2208	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13021	.	stem_loop	2140	2183	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13021	.	engineered_region	1	1371	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_I13021	.	terminator	1180	1308	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_I13021	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13021	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13021	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13021	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13021	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13021	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13021	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13021	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13021	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_I13021	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13021	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13021	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13021	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13021	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13021	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_I13021	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I13021	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I13021	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I13021	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I13021	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_I13021	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13021
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgt
tcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcga
gggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctc
gtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccg
ccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaa
gttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcac
aagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttca
agatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccc
cgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatg
gtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata