##gff-version 3
##sequence-region BBa_I12244 1 3268
BBa_I12244	.	engineered_region	2370	3131	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I12244	.	CDS	2370	3131	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I12244	.	point_mutation	2438	2438	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I12244	.	tag	3088	3125	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I12244	.	stop_codon	3126	3131	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I12244	.	engineered_region	2370	3131	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I12244	.	ribosome_entry_site	2352	2363	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12244	.	conserved_region	2356	2359	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12244	.	promoter	1134	2343	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I12244	.	promoter	2213	2343	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I12244	.	promoter	2176	2247	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I12244	.	sequence_feature	1134	2212	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I12244	.	terminator	3140	3219	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12244	.	engineered_region	3140	3219	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12244	.	stem_loop	3151	3194	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12244	.	terminator	997	1076	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12244	.	engineered_region	997	1076	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12244	.	stem_loop	1008	1051	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12244	.	terminator	1085	1125	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12244	.	polyA_site	1112	1125	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12244	.	stem_loop	1092	1111	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12244	.	engineered_region	1085	1125	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12244	.	stop_codon	1118	1118	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12244	.	terminator	3228	3268	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12244	.	polyA_site	3255	3268	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12244	.	stem_loop	3235	3254	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12244	.	engineered_region	3228	3268	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12244	.	stop_codon	3261	3261	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12244	.	CDS	82	963	.	+	0	ID=BBa_I12205;Name=BBa_I12205
BBa_I12244	.	engineered_tag	964	988	.	+	0	ID=annotation2214023;Name=Help:Barcodes;Parent=BBa_I12205
BBa_I12244	.	CDS	82	963	.	+	0	ID=annotation1032104;Name=araC;Parent=BBa_I12205
BBa_I12244	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I12244	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12244	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I12244	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12244	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12244	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12244	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12244	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12244	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I12244	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12244	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12244	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_I12244
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtact
agatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgat
tgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgc
ggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagaga
ttcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggca
tgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagc
gacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagc
aattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtca
gtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgt
ctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagc
tgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcg
agtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtc
aagttgtcataataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgg
aactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgac
cgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaa
gacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcg
atatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatgga
gcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgccc
ttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaac
cccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgat
accattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggca
aacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagc
ggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagt
atcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtcc
atattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaa
agcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtcc
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactaga
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatga
agcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcaga
agaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata